Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Anal Chim Acta. 2017 Aug 1;979:51-57. doi: 10.1016/j.aca.2017.05.010. Epub 2017 May 26.
Meso/macroporous cobalt (II) oxide nanoflakes were electrodeposited in a one-step process in the presence of N-methylpyrrolidone. On the surface of nanoflakes, a specific single stranded DNA sequence from the genome of influenza A subtype was then immobilized to fabricate an electrochemical biosensor. Hybridization of the biosensor with complementary, non-complementary and base-mismatch sequences was electrochemically detected. The biosensor was also employed to detect complementary DNA of viral RNA in culture and human samples. The biosensor could detect the complementary sequence with a detection limit of 86.4 amol L and a linear concentration range of 1.0 fmol L to 1.0 nmol L. It also detected a complementary DNA sequence converted from viral RNA with a detection limit of 0.28 ng μL in a linear concentration range of 0.5-10 ng μL. Low detection limit, simple method of preparation of the transducer and no needing any DNA strand modification and tag are the principal advantages of the biosensor.
介孔/大孔氧化钴纳米片在 N-甲基吡咯烷酮的存在下通过一步电沉积法进行电沉积。然后，在纳米片的表面固定流感 A 亚型基因组的特定单链 DNA 序列，以制备电化学生物传感器。通过电化学检测生物传感器与互补、非互补和碱基错配序列的杂交。该生物传感器还用于检测培养物和人体样本中的病毒 RNA 的互补 DNA。该生物传感器可以检测互补序列，检测限为 86.4 amol L，线性浓度范围为 1.0 fmol L 至 1.0 nmol L。它还可以检测从病毒 RNA 转化而来的互补 DNA 序列，在 0.5-10 ng μL 的线性浓度范围内检测限为 0.28 ng μL。低检测限、传感器制备方法简单，无需任何 DNA 链修饰和标记是该生物传感器的主要优点。
