Negahdary M, Behjati-Ardakani M, Sattarahmady N, Heli H
Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
J Biomed Phys Eng. 2018 Jun 1;8(2):167-178. eCollection 2018 Jun.
Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.
In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.
The detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. A gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a TnI biosensor.
The designed aptasensor could detect TnI in a range of 0.03 to 2.0 ng mL-1 without using any label, pre-concentration or amplification steps. The limit of detection was attained as 10 pg mL-1 without significant trouble of interfering species. The TnI biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the TnI biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively.
This aptasensor may be used as an applicable tool in the future of early medical diagnosis of MI.
急性心肌梗死（MI）占死亡人数的三分之一。心肌肌钙蛋白I（TnI）是心肌组织损伤的可靠生物标志物，用于MI的早期诊断。
本研究介绍一种通过快速检测TnI对MI进行早期诊断的分子方法。
检测方法基于电化学适体传感，采用不同方法和评估步骤进行开发。使用金电极作为换能器，成功固定76个碱基的适体以制备TnI生物传感器。
所设计的适体传感器无需任何标记、预浓缩或扩增步骤，即可在0.03至2.0 ng mL-1范围内检测TnI。检测限达到10 pg mL-1，且无明显干扰物质问题。TnI生物传感器表现出稳定、可再生和可重复的功能。使用89份人类样本评估TnI生物传感器的性能，其诊断敏感性和特异性分别为100%和81%。
这种适体传感器未来可能成为MI早期医学诊断的适用工具。
