Zhao Zhi, Huang Changfu, Huang Ziyu, Lin Fengjuan, He Qinlin, Tao Dan, Jaffrezic-Renault Nicole, Guo Zhenzhong
Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan 430065, PR China.
School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan 430065, PR China.
Trends Analyt Chem. 2021 Jun;139:116253. doi: 10.1016/j.trac.2021.116253. Epub 2021 Mar 12.
Respiratory viruses are real menace for human health which result in devastating epidemic disease. Consequently, it is in urgent need of identifying and quantifying virus with a rapid, sensitive and precise approach. The study of electrochemical biosensors for respiratory virus detection has become one of the most rapidly developing scientific fields. Recent developments in electrochemical biosensors concerning respiratory virus detection are comprehensively reviewed in this paper. This review is structured along common detecting objects of respiratory viruses, electrochemical biosensors, electrochemical biosensors for respiratory virus detection and future challenges. The electrochemical biosensors for respiratory virus detection are introduced, including nucleic acids-based, immunosensors and other affinity biosensors. Lastly, for Coronavirus disease 2019 (COVID-19) diagnosis, the future challenges regarding developing electrochemical biosensor-based Point-of-Care Tests (POCTs) are summarized. This review is expected to provide a helpful guide for the researchers entering this interdisciplinary field and developing more novel electrochemical biosensors for respiratory virus detection.
呼吸道病毒对人类健康构成了真正的威胁，会引发毁灭性的流行病。因此，迫切需要采用快速、灵敏且精确的方法来识别和定量病毒。用于呼吸道病毒检测的电化学生物传感器研究已成为发展最为迅速的科学领域之一。本文全面综述了电化学生物传感器在呼吸道病毒检测方面的最新进展。本综述按照呼吸道病毒的常见检测对象、电化学生物传感器、用于呼吸道病毒检测的电化学生物传感器以及未来挑战进行架构。介绍了用于呼吸道病毒检测的电化学生物传感器，包括基于核酸的传感器、免疫传感器和其他亲和生物传感器。最后，针对2019冠状病毒病（COVID-19）的诊断，总结了开发基于电化学生物传感器的即时检测（POCT）所面临的未来挑战。本综述有望为进入这一跨学科领域的研究人员以及开发更多用于呼吸道病毒检测的新型电化学生物传感器提供有益的指导。
