Blue Laura E, Franklin Edward G, Godinho Justin M, Grinias James P, Grinias Kaitlin M, Lunn Daniel B, Moore Stephanie M
Process Development, Amgen Inc., Thousand Oaks, CA 91320, USA.
HPLC Research & Development, Restek Corp., Bellefonte, PA 16823, USA.
J Chromatogr A. 2017 Nov 10;1523:17-39. doi: 10.1016/j.chroma.2017.05.039. Epub 2017 May 17.
In the twenty years since its initial demonstration, capillary ultrahigh pressure liquid chromatography (UHPLC) has proven to be one of most powerful separation techniques for the analysis of complex mixtures. This review focuses on the most recent advances made since 2010 towards increasing the performance of such separations. Improvements in capillary column preparation techniques that have led to columns with unprecedented performance are described. New stationary phases and phase supports that have been reported over the past decade are detailed, with a focus on their use in capillary formats. A discussion on the instrument developments that have been required to ensure that extra-column effects do not diminish the intrinsic efficiency of these columns during analysis is also included. Finally, the impact of these capillary UHPLC topics on the field of proteomics and ways in which capillary UHPLC may continue to be applied to the separation of complex samples are addressed.
自首次展示以来的二十年里，毛细管超高压液相色谱（UHPLC）已被证明是分析复杂混合物最强大的分离技术之一。本综述聚焦于2010年以来在提高此类分离性能方面取得的最新进展。描述了毛细管柱制备技术的改进，这些改进带来了具有前所未有的性能的色谱柱。详细介绍了过去十年报道的新型固定相和相载体，重点是它们在毛细管形式中的应用。还讨论了为确保在分析过程中柱外效应不会降低这些色谱柱的固有效率而需要进行的仪器开发。最后，阐述了这些毛细管UHPLC主题对蛋白质组学领域的影响以及毛细管UHPLC可继续应用于复杂样品分离的方式。
