Lam Shing Chung, Sanz Rodriguez Estrella, Haddad Paul R, Paull Brett
ASTech, ARC Training Centre for Portable Analytical Separation Technologies (ASTech), and Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Private Bag 75, Hobart, Tasmania 7001, Australia.
Analyst. 2019 Jun 7;144(11):3464-3482. doi: 10.1039/c9an00329k. Epub 2019 Apr 12.
This review covers advances and applications of open tubular capillary liquid chromatography (OT-LC) over the period 2007-2018. Under the right conditions OT-LC columns have the potential to offer superior column efficiency, higher overall peak capacity, and higher column permeability compared to packed capillary and monolithic columns. However, such advantages are highly dependent upon column format and dimensions, and to date in liquid chromatography the advantages of open tubular format columns have been most widely discussed and applied in the field of proteomics. In this review we have focused on the wider variety of separation mechanisms and applications which can be achieved following the modification of the inner wall of the capillary with a thin-layer stationary phase. In particular the latest advances in stationary phase development and formation, together with new column formats and dimensions are reviewed. Detection options for OT-LC are also discussed and recent advances in this area highlighted. Finally, this review summarises existing applications of OT-LC and illustrates the future potential for this technique.
本综述涵盖了2007年至2018年期间开管毛细管液相色谱（OT-LC）的进展与应用。在合适的条件下，与填充毛细管柱和整体柱相比，OT-LC柱有潜力提供更高的柱效、更高的总峰容量和更高的柱渗透率。然而，这些优势高度依赖于柱的形式和尺寸，并且迄今为止在液相色谱中，开管形式柱的优势在蛋白质组学领域得到了最广泛的讨论和应用。在本综述中，我们重点关注了通过用薄层固定相修饰毛细管内壁可实现的更广泛的分离机制和应用。特别回顾了固定相开发与形成方面的最新进展，以及新的柱形式和尺寸。还讨论了OT-LC的检测选项，并突出了该领域的最新进展。最后，本综述总结了OT-LC的现有应用，并阐述了该技术的未来潜力。
