Profiles of Long Noncoding RNAs in Human Naive and Memory T Cells.

We employed whole-genome RNA-sequencing to profile mRNAs and both annotated and novel long noncoding RNAs (lncRNAs) in human naive, central memory, and effector memory CD4 T cells. Loci transcribing both lineage-specific annotated and novel lncRNA are adjacent to lineage-specific protein-coding genes in the genome. Lineage-specific novel lncRNA loci are transcribed from lineage-specific typical- and supertranscriptional enhancers and are not multiexonic, thus are more similar to enhancer RNAs. Novel enhancer-associated lncRNAs transcribed from the locus bind the transcription factor NF-κB and enhance binding of NF-κB to the genomic locus. Depletion of the annotated lncRNA, IFNG-AS1, or one enhancer-associated lncRNA abrogates expression by memory T cells, indicating these lncRNAs have biologic function.