Wu Yilan, Zhang Yin, Sun Yunpeng, Yu Jiali, Wang Peiliang, Ma Huan, Chen Shijunyin, Ma Lizhen, Zhang Dongyang, He Qun, Guo Jinhu
Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510006, China.
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100083, China.
Genetics. 2017 Aug;206(4):1881-1893. doi: 10.1534/genetics.117.202788. Epub 2017 Jun 9.
Nonsense-mediated RNA decay (NMD) is a crucial post-transcriptional regulatory mechanism that recognizes and eliminates aberrantly processed transcripts, and mediates the expression of normal gene transcripts. In this study, we report that in the filamentous fungus , the NMD factors play a conserved role in regulating the surveillance of NMD targets including premature termination codon (PTC)-containing transcripts and normal transcripts. The circadian rhythms in all of the knockout strains of genes, which encode the Up-frameshift proteins, were aberrant. The knockout strain displays a shortened circadian period, which can be restored by constantly expressing exogenous Up-frameshift protein 1 (UPF1). UPF1 regulates the circadian clock by modulating the splicing of the core clock gene () through spliceosome and spliceosome-related arginine/serine-rich splicing factors, which partly account for the short periods in the knockout strain. We also demonstrated that the clock genes including White Collar (WC)-1, WC-2, and FRQ are involved in controlling the diurnal growth rhythm, and UPF1 may affect the growth rhythms by mediating the FRQ protein levels in the daytime. These findings suggest that the NMD factors play important roles in regulating the circadian clock and diurnal growth rhythms in .
无义介导的RNA降解（NMD）是一种关键的转录后调控机制，它能够识别并消除异常加工的转录本，并介导正常基因转录本的表达。在本研究中，我们报道在丝状真菌中，NMD因子在调控NMD靶标的监测中发挥保守作用，这些靶标包括含有提前终止密码子（PTC）的转录本和正常转录本。所有编码移码上调蛋白的基因敲除菌株的昼夜节律均异常。敲除菌株表现出昼夜周期缩短，持续表达外源移码上调蛋白1（UPF1）可使其恢复。UPF1通过剪接体和与剪接体相关的富含精氨酸/丝氨酸的剪接因子调节核心生物钟基因（）的剪接，这部分解释了敲除菌株中周期缩短的现象。我们还证明，包括白领（WC）-1、WC-2和FRQ在内的生物钟基因参与控制昼夜生长节律，并且UPF1可能通过在白天介导FRQ蛋白水平来影响生长节律。这些发现表明，NMD因子在调节丝状真菌的生物钟和昼夜生长节律中发挥重要作用。
