用于诊断细菌性阴道病的多重肽核酸荧光原位杂交技术（PNA-FISH）

Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis.

作者信息

Machado Antonio, Cerca Nuno

机构信息

Microbiology Institute, Universidad San Francisco de Quito, Diego de Robles y Vía Interoceánica, Cumbayá, Quito, 170157, Ecuador.

CEB-Centre of Biological Engineering, LIBRO-Laboratory of Research in Biofilms Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.

出版信息

Methods Mol Biol. 2017;1616:209-219. doi: 10.1007/978-1-4939-7037-7_13.

DOI:10.1007/978-1-4939-7037-7_13

PMID:28600771

Abstract

Fluorescence in situ hybridization (FISH) is a molecular method used to identify and quantify microorganisms in a wide range of samples. This technique combines the simplicity of microscopic observation and the specificity of DNA/rRNA hybridization, allowing detection of selected bacterial species and morphologic visualization. Here, we describe a quantitative molecular diagnosis of bacterial vaginosis, based on the classical Nugent score. Our probes are able to differentiate Lactobacillus spp. and Gardnerella vaginalis from the other undefined bacterial species considered in the Nugent score.

摘要

荧光原位杂交（FISH）是一种分子方法，用于鉴定和定量各种样本中的微生物。该技术结合了显微镜观察的简便性和DNA/rRNA杂交的特异性，能够检测选定的细菌种类并进行形态可视化。在此，我们描述了一种基于经典 Nugent 评分的细菌性阴道病定量分子诊断方法。我们的探针能够将乳杆菌属和阴道加德纳菌与 Nugent 评分中考虑的其他未定义细菌种类区分开来。