CoRx Life sciences and Pharmaceutical (CLAP) Private Limited, Tiruchirappalli, Tamil Nadu, 620 020, India.
National Repository for Microalgae and Cyanobacteria - Freshwater (DBT), Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India.
Environ Sci Pollut Res Int. 2018 Apr;25(11):10362-10370. doi: 10.1007/s11356-017-9367-9. Epub 2017 Jun 9.
Of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (AgNPs) received great attention due to their versatile properties. In this report, Daucus carota extract was used for the synthesis of AgNPs and ceftriaxone was conjugated with AgNPs to enhance their antimicrobial efficacy. The conjugated and unconjugated AgNPs were characterized by adopting UV-Vis spectroscopy, FTIR, AFM, DLS, and TEM, which revealed the SPR peak at 420 nm and spherical shaped nanoparticles of 20 nm size, respectively. The antimicrobial efficacies of the unconjugated AgNPs and ceftriaxone-conjugated AgNPs were tested against ceftriaxone-resistant human pathogens, Bacillus cereus, Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The ceftriaxone-conjugated AgNPs showed high inhibitory action (23 mm) than the unconjugated AgNPs (18 mm) at the concentration of 50 μg/mL. Both the unconjugated and ceftriaxone-conjugated AgNPs were found to be non-toxic on EAC cells at 50 μg/mL. The dose-dependent cytotoxic activities were observed on increasing the concentration of the AgNPs. The ceftriaxone-conjugated AgNPs showed high activity than the unconjugated AgNPs. The enhanced activity could be useful to treat ceftriaxone-resistant human pathogens.
在探索合成纳米粒子的各种方法中，由于银纳米粒子（AgNPs）具有多种特性，因此其生物合成受到了极大的关注。在本报告中，使用胡萝卜提取物来合成 AgNPs，并将头孢曲松与 AgNPs 缀合以提高其抗菌功效。通过采用 UV-Vis 光谱法、FTIR、AFM、DLS 和 TEM 对缀合和未缀合的 AgNPs 进行了表征，分别在 420nm 处显示出 SPR 峰和 20nm 大小的球形纳米粒子。未缀合的 AgNPs 和头孢曲松缀合的 AgNPs 的抗菌功效针对头孢曲松耐药的人类病原体，蜡样芽孢杆菌，金黄色葡萄球菌，肺炎克雷伯菌和铜绿假单胞菌进行了测试。头孢曲松缀合的 AgNPs 在 50μg/mL 的浓度下显示出比未缀合的 AgNPs（18mm）更高的抑制作用（23mm）。在 50μg/mL 时，未缀合和头孢曲松缀合的 AgNPs 均被发现对 EAC 细胞无毒性。随着 AgNPs 浓度的增加，观察到剂量依赖性的细胞毒性活性。头孢曲松缀合的 AgNPs 比未缀合的 AgNPs 具有更高的活性。这种增强的活性可能有助于治疗头孢曲松耐药的人类病原体。
