利用从黄果藤黄中分离出的内生细菌蜡样芽孢杆菌生物合成抗菌银纳米颗粒。
Biogenesis of antibacterial silver nanoparticles using the endophytic bacterium Bacillus cereus isolated from Garcinia xanthochymus.
作者信息
Sunkar Swetha, Nachiyar C Valli
机构信息
Department of Biotechnology, Sathyabama University, Chennai, India.
出版信息
Asian Pac J Trop Biomed. 2012 Dec;2(12):953-9. doi: 10.1016/S2221-1691(13)60006-4.
OBJECTIVE
To synthesize the ecofriendly nanoparticles, which is viewed as an alternative to the chemical method which initiated the use of microbes like bacteria and fungi in their synthesis.
METHODS
The current study uses the endophytic bacterium Bacillus cereus isolated from the Garcinia xanthochymus to synthesize the silver nanoparticles (AgNPs). The AgNPs were synthesized by reduction of silver nitrate solution by the endophytic bacterium after incubation for 3-5 d at room temperature. The synthesis was initially observed by colour change from pale white to brown which was confirmed by UV-Vis spectroscopy. The AgNPs were further characterized using FTIR, SEM-EDX and TEM analyses.
RESULTS
The synthesized nanoparticles were found to be spherical with the size in the range of 20-40 nm which showed a slight aggregation. The energy-dispersive spectra of the nanoparticle dispersion confirmed the presence of elemental silver. The AgNPs were found to have antibacterial activity against a few pathogenic bacteria like Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae.
CONCLUSIONS
The endophytic bacteria identified as Bacillus cereus was able to synthesize silver nanoparticles with potential antibacterial activity.
目的
合成生态友好型纳米颗粒，将其视为化学方法的替代方法，化学方法开始利用细菌和真菌等微生物进行合成。
方法
本研究使用从山竹中分离出的内生细菌蜡样芽孢杆菌来合成银纳米颗粒（AgNPs）。通过内生细菌在室温下孵育3 - 5天后还原硝酸银溶液来合成AgNPs。最初通过颜色从浅白色变为棕色来观察合成过程，这通过紫外可见光谱法得到证实。使用傅里叶变换红外光谱（FTIR）、扫描电子显微镜 - 能谱分析（SEM - EDX）和透射电子显微镜（TEM）分析对AgNPs进行进一步表征。
结果
合成的纳米颗粒呈球形，尺寸在20 - 40纳米范围内，有轻微聚集现象。纳米颗粒分散体的能量色散光谱证实了元素银的存在。发现AgNPs对一些病原菌如大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌、伤寒沙门氏菌和肺炎克雷伯菌具有抗菌活性。
结论
鉴定为蜡样芽孢杆菌的内生细菌能够合成具有潜在抗菌活性的银纳米颗粒。
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