Viudes-de-Castro María Pilar, Marco-Jiménez Francisco, Cedano-Castro José Isaí, Vicente José Salvador
Centro de Investigación y Tecnología Animal (CITA), Instituto Valenciano de Investigaciones Agrarias (IVIA), 12400 Segorbe, Castellón, Spain.
Institute of Science and Animal Technology (ICTA), Universitat Politècnica de València, 46022 Valencia, Spain.
Theriogenology. 2017 Aug;98:68-74. doi: 10.1016/j.theriogenology.2017.05.005. Epub 2017 May 4.
There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable embryos per donor, in order to either support genetic improvement programs, ex situ conservation or to optimize other biotechnologies. Over time, the use of this biotechnology has shown variable outcomes as a consequence of several factors, such as the origin of exogenous hormone, posology and the effect of gonadotropins used simultaneously, the donor and the environment. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (CTP), alone or supplemented with LH, versus a FSH standard protocol of five equal doses administered twice daily to superovulate rabbit does (20 per group and 29 control females). We determined: 1) the impact of this stimulation on in vitro development and mRNA expression at blastocyst stage and 2) in vivo embryo development and viability rate at birth of transferred embryos. Our outcomes showed that the ovulation rate was similar among the different ovarian stimulation groups, reaching more than fourfold the ovulation rate of a control doe. While rates of embryos developing to the blastocyst stage after 48 h of in vitro culture were similar between groups, the hatched blastocyst rate was higher for superovulated embryos from CTP group. Moreover, no significant differences among mRNA expression of OCT4, SOX2 and NANOG genes were detected. Nevertheless, embryos from ovarian stimulated does with CTP + LH showed significantly higher implantation rates and survival at birth among the different ovarian stimulation groups and similar to those in the control group. In conclusion, the results of this study suggest that a single injection of long acting corifollitropin alfa can be effectively used in rabbits to elicit a more than fourfold increase in ovulation rate compared to control animals. In addition, the LH supplementation allows us to obtain similar in vivo embryo development results as in the control group.
将兔子用作实验室模型以及用于肉类、羊毛和皮毛的工业生产，正受到越来越多的关注。动物超排卵用于使每个供体产生最大数量的可移植胚胎，以支持遗传改良计划、迁地保护或优化其他生物技术。随着时间的推移，由于多种因素，如外源激素的来源、剂量以及同时使用的促性腺激素的影响、供体和环境等，这种生物技术的使用显示出不同的结果。本研究的目的是比较单次注射阿法科里福美（CTP）单独使用或补充LH与每天两次给予五个等量剂量的FSH标准方案对兔母兔进行超排卵的效果（每组20只，29只对照雌性）。我们确定了：1）这种刺激对囊胚阶段体外发育和mRNA表达的影响，以及2）体内胚胎发育和移植胚胎出生时的存活率。我们的结果表明，不同卵巢刺激组的排卵率相似，达到对照母兔排卵率的四倍以上。虽然体外培养48小时后发育到囊胚阶段的胚胎率在各组之间相似，但CTP组超排卵胚胎的孵化囊胚率更高。此外，未检测到OCT4、SOX2和NANOG基因的mRNA表达有显著差异。然而，在不同卵巢刺激组中，用CTP + LH刺激卵巢的母兔的胚胎着床率和出生时的存活率显著更高，且与对照组相似。总之，本研究结果表明，单次注射长效阿法科里福美可有效地用于兔子，与对照动物相比可使排卵率提高四倍以上。此外，补充LH使我们能够获得与对照组相似的体内胚胎发育结果。
