Reina Jordi, Morales Carmen, Busquets María, Norte Cristina
Unidad de Virología, Servicio de Microbiología, Hospital Universitario Son Espases, Palma de Mallorca, España.
Unidad de Virología, Servicio de Microbiología, Hospital Universitario Son Espases, Palma de Mallorca, España.
Enferm Infecc Microbiol Clin (Engl Ed). 2018 Jun-Jul;36(6):332-335. doi: 10.1016/j.eimc.2017.04.008. Epub 2017 Jun 7.
Acute respiratory infections of viral cause are very frequent entities. The difficulty in evaluating the detection of a virus in these entities could be solved by determining the viral load.
A prospective study on the mean Ct value (cycle threshold value) detected against RSV-A, RSV-B and influenza A (H1N1)pdm09, A (H3N2) and B viruses in patients of different origin and age was performed. Detection was performed using a commercial molecular amplification (RT-PCR) technique.
Different mean Ct values were detected for each virus. In RSV infections, no differences were observed between those caused by RSV-A or RSV-B in children. Depending on the patient's age, the only statistical significance was observed in those included in the 0-4 month groups for RSV-A and this group and the 5-12 months group for RSV-B (higher values). A lower viral load was detected in adult patients than in paediatric patients. In influenza infections, no statistical significance was observed in the mean values detected in patients from the Red Centinela («sentinel network», a Spanish network of doctors aimed at research and surveillance of diseases), those diagnosed in the adult emergency room or in hospital admissions. In the adult patients admitted to the ICU, only a slightly lower mean value was observed in those infected with influenza A (H1N1)pdm09, but without statistical significance. There were no patients admitted to the ICU with influenza B infection.
The detection of viral load could be a good tool for the evaluation, monitoring and prognosis of acute viral respiratory infections. With the exception of those caused by RSV, no significant differences were observed in influenza infections except in younger paediatric patients.
由病毒引起的急性呼吸道感染是非常常见的病症。通过测定病毒载量可以解决评估这些病症中病毒检测的难题。
对不同来源和年龄的患者针对呼吸道合胞病毒A（RSV-A）、呼吸道合胞病毒B（RSV-B）以及甲型流感（H1N1）pdm09、甲型流感（H3N2）和乙型流感病毒检测到的平均Ct值（循环阈值）进行了一项前瞻性研究。检测使用商业分子扩增（RT-PCR）技术。
每种病毒检测到的平均Ct值不同。在呼吸道合胞病毒感染中，儿童中由RSV-A或RSV-B引起的感染之间未观察到差异。根据患者年龄，仅在0至4个月组的RSV-A患者以及该组和5至12个月组的RSV-B患者中观察到统计学意义（值更高）。成年患者检测到的病毒载量低于儿科患者。在流感感染中，在红哨兵网络（“哨兵网络”，西班牙旨在疾病研究和监测的医生网络）的患者、在成人急诊室诊断的患者或住院患者中检测到的平均值未观察到统计学意义。在入住重症监护病房（ICU）的成年患者中，仅在感染甲型流感（H1N1）pdm09的患者中观察到略低的平均值，但无统计学意义。没有乙型流感感染的患者入住ICU。
病毒载量检测可能是评估、监测和预测急性病毒性呼吸道感染的良好工具。除了由呼吸道合胞病毒引起的数据外，流感感染中除了较年幼的儿科患者外未观察到显著差异。
