创伤性脑损伤会损害小鼠的感觉运动功能。
Traumatic brain injury impairs sensorimotor function in mice.
作者信息
Sackheim Adrian M, Stockwell David, Villalba Nuria, Haines Laurel, Scott Chary L, Russell Sheila, Hammack Sayamwong E, Freeman Kalev
机构信息
Department of Surgery, University of Vermont, Burlington, Vermont.
Department of Surgery, University of Vermont, Burlington, Vermont; Department of Pharmacology, University of Vermont, Burlington, Vermont.
出版信息
J Surg Res. 2017 Jun 1;213:100-109. doi: 10.1016/j.jss.2017.02.016. Epub 2017 Feb 23.
INTRODUCTION
Understanding the extent to which murine models of traumatic brain injury (TBI) replicate clinically relevant neurologic outcomes is critical for mechanistic and therapeutic studies. We determined sensorimotor outcomes in a mouse model of TBI and validated the use of a standardized neurologic examination scoring system to quantify the extent of injury.
MATERIALS AND METHODS
We used a lateral fluid percussion injury model of TBI and compared TBI animals to those that underwent sham surgery. We measured neurobehavioral deficits using a standardized 12-point neurologic examination, magnetic resonance imaging, a rotating rod test, and longitudinal acoustic startle testing.
RESULTS
TBI animals had a significantly decreased ability to balance on a rotating rod and a marked reduction in the amplitude of acoustic startle response. The neurologic examination had a high inter-rater reliability (87% agreement) and correlated with latency to fall on a rotating rod (R = -0.809).
CONCLUSIONS
TBI impairs sensorimotor function in mice, and the extent of impairment can be predicted by a standardized neurologic examination.
引言
了解创伤性脑损伤（TBI）小鼠模型在多大程度上复制临床相关神经学结果对于机制研究和治疗研究至关重要。我们确定了TBI小鼠模型中的感觉运动结果，并验证了使用标准化神经学检查评分系统来量化损伤程度。
材料与方法
我们使用了TBI的侧方流体冲击伤模型，并将TBI动物与接受假手术的动物进行比较。我们使用标准化的12分神经学检查、磁共振成像、转棒试验和纵向听觉惊吓试验来测量神经行为缺陷。
结果
TBI动物在转棒上保持平衡的能力显著下降，听觉惊吓反应的幅度明显降低。神经学检查具有较高的评分者间信度（一致性为87%），并与在转棒上跌倒的潜伏期相关（R = -0.809）。
结论
TBI损害小鼠的感觉运动功能，损伤程度可通过标准化神经学检查预测。
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