Ali Imran, Jan Mehmood, Wakeel Abdul, Azizullah Azizullah, Liu Bohan, Islam Faisal, Ali Abid, Daud M K, Liu Yihua, Gan Yinbo
Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China; Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan.
Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Ecotoxicol Environ Saf. 2017 Oct;144:62-71. doi: 10.1016/j.ecoenv.2017.06.015. Epub 2017 Jun 8.
Bisphenol A (BPA), an important raw material in plastic industry, has become a serious environmental contaminant due to its wide spread use in different products and increasing release into the environment. BPA is known to cause adverse effects in living organisms including plants. Several studies reported that BPA affects growth and development in plants, mainly through oxidative stress. Plants are known to generally cope with stress mainly through hormonal regulation and adaptation, but little is known about the role of plant hormones in plants under BPA stress. The present study was conducted to investigate the role of ethylene in BPA induced oxidative stress in plants using Arabidopsis thaliana as a test plant. The response of ethylene insensitive mutants of Arabidopsis (ein2-1 and etr1-3) to BPA exposure was studied in comparison to the wild type Arabidopsis (WT). In all three genotypes, exposure to BPA adversely affected cellular structures, stomata and light-harvesting pigments. An increase in reactive oxygen species (ROS) lipid peroxidation and other oxidative stress markers indicated that BPA induced toxicity through oxidative stress. However, the overall results revealed that WT Arabidopsis had more pronounced BPA induced damages while ein2-1 and etr1-3 mutants withstood the BPA induced stress more efficiently. The activity of antioxidant enzymes and expression of antioxidants related genes revealed that the antioxidant defense system in both mutants was more efficiently activated than in WT against BPA induced oxidative stress, which further evidenced the involvement of ethylene in regulating BPA induced oxidative stress. It is concluded that ethylene perception and signaling may be involved in BPA induced oxidative stress responses in plants.
双酚A（BPA）是塑料工业中的一种重要原料，由于其在不同产品中的广泛使用以及向环境中的释放量不断增加，已成为一种严重的环境污染物。已知双酚A会对包括植物在内的生物有机体产生不利影响。多项研究报告称，双酚A主要通过氧化应激影响植物的生长和发育。已知植物通常主要通过激素调节和适应来应对胁迫，但关于植物激素在双酚A胁迫下的植物中的作用知之甚少。本研究以拟南芥为试验植物，旨在研究乙烯在双酚A诱导的植物氧化应激中的作用。将拟南芥乙烯不敏感突变体（ein2-1和etr1-3）与野生型拟南芥（WT）相比，研究它们对双酚A暴露的反应。在所有三种基因型中，暴露于双酚A均对细胞结构、气孔和光捕获色素产生不利影响。活性氧（ROS）、脂质过氧化和其他氧化应激标志物的增加表明，双酚A通过氧化应激诱导毒性。然而，总体结果显示，野生型拟南芥受到的双酚A诱导损伤更为明显，而ein2-1和etr1-3突变体对双酚A诱导的胁迫具有更强的耐受性。抗氧化酶活性和抗氧化相关基因的表达表明，与野生型相比，两种突变体中的抗氧化防御系统在应对双酚A诱导的氧化应激时被更有效地激活，这进一步证明了乙烯参与调节双酚A诱导的氧化应激。研究得出结论，乙烯感知和信号传导可能参与植物对双酚A诱导的氧化应激反应。
