da Silva A R M, Farias M L, da Silva D L S, Vitoriano J O, de Sousa R C, Alves-Junior C
LABPLASMA- Department of Exact and Natural Sciences, Federal Rural University of Semiarid, Mossoró, RN, CEP: 59625-900 Brazil.
LCMM - Physics Department, Federal University of Maranhão São Luís, MA, CEP: 65080-805, Brazil.
Colloids Surf B Biointerfaces. 2017 Sep 1;157:280-285. doi: 10.1016/j.colsurfb.2017.05.063. Epub 2017 Jun 1.
In this study, we analyzed seed wettability as well as imbibition and germination after treatment with atmospheric pressure cold plasma (APCP) using dielectric barrier discharge (DBD) in seeds that have very low germination rates. To aid industrial applications, several seeds were simultaneously treated with plasma within a space between two coaxial glass tubes sandwiched by two metal mesh screens that produced high-voltage pulses at 17.5kV with a frequency of 990Hz. Three treatment times (3min, 9min and 15min) as well as untreated seeds were used to conduct the wettability, imbibition and germination tests. The wettability and imbibition were found to be directly related to the treatment duration, but saturation of the imbibition was found for treatment durations greater than 9min. Plasma treatment was also effective in improving germination, but shorter treatment duration presented greater germination. This apparent contradiction is explained by the cell damage caused by the increased exposure to plasma, as observed in other studies. The results suggest that there must be an optimal wettability and imbibition condition that ensures that excessive moisture does not harm the germination process.
在本研究中，我们分析了常压冷等离子体（APCP）处理后种子的润湿性以及吸胀和萌发情况，该常压冷等离子体采用介质阻挡放电（DBD），处理对象为发芽率极低的种子。为助力工业应用，在两根同轴玻璃管之间的空间内，用夹在两个金属网筛之间的装置对多粒种子同时进行等离子体处理，该装置能产生17.5kV、频率为990Hz的高压脉冲。采用三个处理时间（3分钟、9分钟和15分钟）以及未处理的种子进行润湿性、吸胀和萌发试验。发现润湿性和吸胀与处理持续时间直接相关，但处理持续时间大于9分钟时吸胀达到饱和。等离子体处理在促进萌发方面也有效，但较短的处理持续时间萌发效果更佳。正如其他研究所观察到的，这种明显的矛盾可以通过等离子体暴露增加导致的细胞损伤来解释。结果表明，必须存在一个最佳的润湿性和吸胀条件，以确保过量水分不会损害萌发过程。
