Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, U.P., India.
Plant Physiology Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002, U.P., India.
J Biotechnol. 2017 Nov 20;262:11-27. doi: 10.1016/j.jbiotec.2017.09.016. Epub 2017 Sep 28.
Study on the ecological effect of metal oxide nanomaterials (NMs) has quickly amplified over the precedent years because it is assumed that these NMs will sooner or later be released into the environment. The present study deals with biologically oriented process for the green synthesis of copper oxide nanoparticles (CuO NPs) by using Morus alba leaf extract as reducing agent. Powder X-ray diffraction (XRD) and transmission electron microscope (TEM) analysis revealed the monoclinic phase and 20-40nm size respectively. The presence of reducing and capping agents revealed by Fourier transform infrared (FTIR) spectroscopy. The seedlings of Brassica oleracea var. botrytis and Solanum lycopersicum were exposed to 10, 50, 100, and 500mgL concentrations of CuO NPs in the sand medium. Bioaccumulation of Cu was also investigated by atomic absorption spectroscopy (AAS). Plant exposure to 100 and 500mgL of CuO NPs has resulted in significant reduction of total chlorophyll and sugar content in the two test plants while 10mgL of NPs slightly increased the pigment and sugar content in tomato plants only. Augmentation of lipid peroxidation, electrolyte leakage, and antioxidant enzyme activity was observed in a dose dependent manner upon plants exposure to CuO NPs. Deposition of lignin in roots of both plants treated with the highest concentration of CuO NPs was observed. Histochemical analysis of leaves of treated plant with nitroblue tetrazolium and 33 diaminobenzidine showed a concentration dependent increase in superoxide and hydrogen peroxide formation in leaves. The green synthesis of CuO NPs was carried out by using Morus alba leaf extract. Accumulation of NPs more actively by tomato plants as compared to cauliflower was possibly due to the difference in root morphology. The histochemical visualization highlights the spatial organization of oxidant biochemistry occurring in response to metal stress.
金属氧化物纳米材料（NMs）的生态效应研究在过去几年中迅速增加，因为人们认为这些 NMs 迟早会释放到环境中。本研究采用桑树叶片提取物作为还原剂，进行了生物导向的氧化铜纳米颗粒（CuO NPs）的绿色合成。粉末 X 射线衍射（XRD）和透射电子显微镜（TEM）分析分别显示出单斜相和 20-40nm 的尺寸。傅里叶变换红外（FTIR）光谱显示了还原剂和封端剂的存在。用 Brassica oleracea var. botrytis 和 Solanum lycopersicum 的幼苗在沙质培养基中暴露于 10、50、100 和 500mgL 的 CuO NPs 浓度下。原子吸收光谱（AAS）也研究了 Cu 的生物积累。暴露于 100 和 500mgL 的 CuO NPs 的植物导致两种测试植物的总叶绿素和糖含量显著降低，而 10mgL 的 NPs 仅略微增加番茄植物的色素和糖含量。植物暴露于 CuO NPs 时，脂质过氧化、电解质渗漏和抗氧化酶活性呈剂量依赖性增加。在两种植物的根中观察到木质素的沉积。用氮蓝四唑和 33 二氨基联苯胺对处理植物的叶片进行组织化学分析，显示叶片中超氧化物和过氧化氢的形成呈浓度依赖性增加。CuO NPs 的绿色合成是通过使用桑树叶片提取物进行的。与花椰菜相比，番茄植物对 NPs 的积累更活跃，这可能是由于根系形态的差异。组织化学可视化突出了金属胁迫下发生的氧化剂生物化学的空间组织。
