用于采集特定猪神经节的简单且可重现的方法。
Simple and reproducible approaches for the collection of select porcine ganglia.
机构信息
Department of Pathology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
出版信息
J Neurosci Methods. 2017 Sep 1;289:93-98. doi: 10.1016/j.jneumeth.2017.06.005. Epub 2017 Jun 8.
BACKGROUND
The anatomy and physiology of the pig nervous system is more similar to humans compared to traditional rodent models. This makes the pig an attractive model to answer questions relating to human health and disease. Yet the technical and molecular tools available to pig researchers are limited compared to rodent researchers.
NEW METHOD
We developed simple and rapid methods to isolate the trigeminal, nodose (distal vagal), and dorsal root ganglia from neonatal pigs. We selected these ganglia due to their broad applicability to basic science researchers and clinicians.
RESULTS
Use of these methods resulted in reproducible isolation of all three types of ganglia as validated by histological examination.
COMPARISON WITH EXISTING METHOD(S): There are currently no methods that describe a step-by-step protocol to isolate these porcine ganglia.
CONCLUSIONS
In conclusion, these methods for ganglia collection will facilitate and accelerate future neuroscience investigations in pig models of human disease.
背景
与传统的啮齿动物模型相比，猪的神经系统解剖结构和生理学更类似于人类。这使得猪成为一种有吸引力的模型，可以回答与人类健康和疾病相关的问题。然而，与啮齿动物研究人员相比，猪研究人员可用的技术和分子工具是有限的。
新方法
我们开发了简单快速的方法从新生猪中分离三叉神经、结状神经节（远侧迷走神经）和背根神经节。我们选择这些神经节是因为它们广泛适用于基础科学研究人员和临床医生。
结果
通过组织学检查验证，这些方法可重复性地分离出所有三种类型的神经节。
与现有方法的比较
目前没有描述逐步分离这些猪神经节的方法。
结论
总之，这些神经节收集方法将促进和加速人类疾病猪模型中未来的神经科学研究。
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