Ziegler Amanda, Gonzalez Liara, Blikslager Anthony
Center for Gastrointestinal Biology and Disease, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina.
Cell Mol Gastroenterol Hepatol. 2016 Nov;2(6):716-724. doi: 10.1016/j.jcmgh.2016.09.003.
Gastrointestinal disease is a prevalent cause of morbidity and mortality and the use of animal models have been instrumental in studying mechanisms of digestive pathophysiology. As investigators attempt to translate the wealth of basic science information developed from rodent, models, large animal models provide a number of translational advantages. The pig, in particular, is arguably one of the most powerful models of human organ systems, including the gastrointestinal tract. The pig has provided important tools and insight into intestinal ischemia/reperfusion injury, intestinal mucosal repair, as well as new insights into esophageal injury and repair. Porcine model development has taken advantage of the size of the animal, allowing increased surgical and endoscopic access. In addition, cellular tools such as the intestinal porcine epithelial cell line and porcine enteroids are providing the methodology to translate basic science findings using in-depth mechanistic analyses. Further opportunities in porcine digestive disease modeling include developing additional transgenic pig strains. Collectively, porcine models hold great promise for the future of clinically relevant digestive disease research.
胃肠疾病是发病和死亡的常见原因，动物模型的使用对研究消化病理生理学机制起到了重要作用。随着研究人员试图将从啮齿动物模型中获得的大量基础科学信息进行转化，大型动物模型具有诸多转化优势。特别是猪，可以说是人类器官系统（包括胃肠道）最强大的模型之一。猪为肠道缺血/再灌注损伤、肠黏膜修复以及食管损伤和修复提供了重要工具和见解。猪模型的开发利用了动物的体型，增加了手术和内镜检查的可及性。此外，诸如猪肠道上皮细胞系和猪肠类器官等细胞工具，为通过深入的机制分析转化基础科学研究结果提供了方法。猪消化系统疾病建模的更多机会包括开发更多的转基因猪品系。总体而言，猪模型在临床相关消化系统疾病研究的未来具有巨大潜力。
