Tsukiyama Yorika, Ito Tatsuo, Nagaoka Kenjiro, Eguchi Eri, Ogino Keiki
Department of Public Health, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
J Clin Biochem Nutr. 2017 May;60(3):180-186. doi: 10.3164/jcbn.16-108. Epub 2017 Apr 7.
The relationship between exercise training and nitric oxide-related parameters was examined in a cross-sectional study and an intervention study. A cross-sectional study using 184 employees was conducted to observe the association of exercise habits with serum arginase (ELISA and activity), l-arginine, l-citrulline, l-ornithine, NOx, exhaled nitric oxide, blood pressure, FEV1%, hs-CRP, HDL-cholesterol, IgE, and life style factors. An intervention study was also conducted to evaluate the changes of serum arginase I, nitric oxide-related parameters, and mRNA levels of anti-oxidant enzymes in blood monocytes before and after 1 h of aerobic exercise training per day for a month. Exercise habits were associated with increased arginase activity and a moderate alcohol drinking habit, after adjustment with several covariates. Aerobic exercise training induced a decrease in l-arginine and diastolic blood pressure and induced an increase in NO2 and urea. Moreover, mRNA expression of anti-oxidant enzymes, such as catalase and GPX1, and a life elongation enzyme, SIRT3, were significantly increased after aerobic exercise. The results that aerobic exercise training increased NO generation, reduced blood pressure, and induced anti-oxidant enzymes via SIRT3 suggest that exercise training may be an important factor for the prevention of disease by inducing intrinsic NO and anti-oxidant enzymes.
在一项横断面研究和一项干预研究中，对运动训练与一氧化氮相关参数之间的关系进行了研究。采用184名员工进行横断面研究，以观察运动习惯与血清精氨酸酶（酶联免疫吸附测定法和活性）、L-精氨酸、L-瓜氨酸、L-鸟氨酸、氮氧化物、呼出一氧化氮、血压、第一秒用力呼气量百分比、超敏C反应蛋白、高密度脂蛋白胆固醇、免疫球蛋白E以及生活方式因素之间的关联。还进行了一项干预研究，以评估每天进行1小时有氧运动训练，持续一个月前后，血液单核细胞中血清精氨酸酶I、一氧化氮相关参数以及抗氧化酶mRNA水平的变化。在对多个协变量进行调整后，运动习惯与精氨酸酶活性增加以及适度饮酒习惯相关。有氧运动训练导致L-精氨酸和舒张压降低，以及二氧化氮和尿素增加。此外，有氧运动后，过氧化氢酶和谷胱甘肽过氧化物酶1等抗氧化酶以及寿命延长酶SIRT3的mRNA表达显著增加。有氧运动训练通过SIRT3增加一氧化氮生成、降低血压并诱导抗氧化酶的结果表明，运动训练可能是通过诱导内源性一氧化氮和抗氧化酶来预防疾病的重要因素。
