印度尼西亚感染的流行情况及分子流行病学
Prevalence and molecular epidemiology of infection in Indonesia.
作者信息
Collins D A, Gasem M H, Habibie T H, Arinton I G, Hendriyanto P, Hartana A P, Riley T V
机构信息
School of Medical & Health Sciences, Edith Cowan University, Perth, Australia.
Department of Medicine, Faculty of Medicine Diponegoro University-Dr. Kariadi Hospital, Semarang, Indonesia.
出版信息
New Microbes New Infect. 2017 May 4;18:34-37. doi: 10.1016/j.nmni.2017.04.006. eCollection 2017 Jul.
has not been studied in detail in Asia, particularly Southeast Asia. We thus performed a prevalence study across four hospitals in Central Java province, Indonesia. Stool samples were collected from patients with diarrhoea and tested by enzyme immunoassay for glutamate dehydrogenase (GDH) and toxin A/B (C DIFF QUIK CHEK COMPLETE, TechLab). Specimens were cultured and molecular typing was performed. In total, 340 samples were tested, of which 70 (20.6%) were GDH positive, with toxin detected in 19 (5.6%). Toxigenic was isolated from 37 specimens (10.9%), while a further 36 (10.6%) nontoxigenic isolates were identified. The most common strain was ribotype 017 (24.3% of 74 isolates), followed by nontoxigenic types QX 224 (9.5%), and QX 238 and QX 108 (both 8.1%). The high prevalence of highlights a need for ongoing surveillance of infection in Indonesia.
在亚洲，尤其是东南亚地区，尚未对此进行详细研究。因此，我们在印度尼西亚中爪哇省的四家医院开展了一项患病率研究。从腹泻患者中采集粪便样本，采用酶免疫分析法检测谷氨酸脱氢酶（GDH）和毒素A/B（艰难梭菌快速检测试剂盒，TechLab公司）。对样本进行培养并进行分子分型。总共检测了340份样本，其中70份（20.6%）GDH呈阳性，19份（5.6%）检测到毒素。从37份标本（10.9%）中分离出产毒菌株，另外鉴定出36份（10.6%）非产毒菌株。最常见的菌株是核糖体分型017（74株分离株中的24.3%），其次是非产毒型QX 224（9.5%），以及QX 238和QX 108（均为8.1%）。艰难梭菌的高患病率表明在印度尼西亚需要持续监测艰难梭菌感染情况。
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