同时检测谷氨酸脱氢酶和毒素 A/B：C. DIFF QUIK CHEK COMPLETE 和 RIDASCREEN 检测方法的比较。
Simultaneous Detection of Glutamate Dehydrogenase and Toxin A/B: Comparison of the C. DIFF QUIK CHEK COMPLETE and RIDASCREEN Assays.
机构信息
Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
出版信息
Ann Lab Med. 2019 Mar;39(2):214-217. doi: 10.3343/alm.2019.39.2.214.
Various commercial assays have recently been developed for detecting glutamate dehydrogenase (GDH) and/or toxin A/B to diagnose infection (CDI). We compared the performance of two assays for the simultaneous detection of GDH and toxin A/B, using 150 stool samples: C. DIFF QUIK CHEK COMPLETE (QCC; TechLab, Blacksburg, VA, USA) and RIDASCREEN GDH (RC-GDH) and Toxin A/B (RC-Toxin A/B; R-Biopharm, Darmstadt, Germany). For GDH detection, QCC and RC-GDH showed satisfactory sensitivity (95.7% and 94.3%, respectively) and specificity (92.5% and 93.8%, respectively) compared with culture. For toxin A/B detection, QCC showed higher sensitivity than RC-Toxin A/B (60.0% vs 33.3%, <0.001) compared with toxigenic culture. When the results of QCC or RC-GDH+RC-Toxin A/B were used as the first step of a two-step algorithm for diagnosing CDI, QCC permitted more accurate discrimination than RC of positive or negative results for CDI (77.3% and 65.3%, respectively). QCC is useful for the simultaneous detection of GDH and toxin A/B as a part of the two-step algorithm for diagnosing CDI.
最近已经开发了各种商业检测方法来检测谷氨酸脱氢酶 (GDH) 和/或毒素 A/B，以诊断 感染 (CDI)。我们比较了两种同时检测 GDH 和毒素 A/B 的检测方法的性能，使用了 150 份粪便样本：C. DIFF QUIK CHEK COMPLETE (QCC; TechLab, Blacksburg, VA, USA) 和 RIDASCREEN GDH (RC-GDH) 和 Toxin A/B (RC-Toxin A/B; R-Biopharm, Darmstadt, Germany)。对于 GDH 检测，QCC 和 RC-GDH 与培养物相比具有令人满意的敏感性（分别为 95.7%和 94.3%）和特异性（分别为 92.5%和 93.8%）。对于毒素 A/B 检测，QCC 比 RC-Toxin A/B 具有更高的敏感性（60.0% vs 33.3%，<0.001），与产毒培养物相比。当 QCC 或 RC-GDH+RC-Toxin A/B 的结果用作诊断 CDI 的两步算法的第一步时，QCC 比 RC 更能准确区分 CDI 的阳性或阴性结果（分别为 77.3%和 65.3%）。QCC 可用于同时检测 GDH 和毒素 A/B，作为诊断 CDI 的两步算法的一部分。
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