Liu Shufen, Sun Yueli, Li Ji, Dong Jingcheng, Bian Qin
Longhua Hospital, Shanghai University of Traditional Chinese Medicine.
The Academy of Integrative Medicine of Fudan University.
J Vis Exp. 2017 May 31(123):55654. doi: 10.3791/55654.
Traditional herbal medicine, an alternative medicine in the clinical setting, has received increased attention in recent years. Before delivery to the body, an additional extraction procedure is commonly required to release the active constituents from raw herbs. Water decoction is a classical extraction procedure that is still broadly used in the clinical settings. Here, we propose a detailed protocol for er-xian decoction (EXD) in order to apply herbal decoctions to experimental studies. The calculation of an animal-appropriate dose is described, as well as the four main steps of EXD: soaking, water decoction, filtration, and concentration. In addition, serum-containing EXD is introduced to rats as a means of in vitro validation. Here, rats were orally administered EXD for three days. Blood samples were then collected, inactivated, centrifuged, and filtered. The serum, diluted with the culture medium, can be utilized to treat cells or tissues in vitro. For example, EXD was applied to both in vivo and in vitro studies and demonstrated that EXD enhances osteogenesis. This protocol can be used as a reference for the preparation and application of herbal medicines.
传统草药医学作为临床环境中的替代医学，近年来受到了越来越多的关注。在输送到人体之前，通常需要额外的提取程序以从生药中释放出活性成分。水煎煮是一种经典的提取程序，目前仍广泛应用于临床环境中。在此，我们提出了一个关于二仙汤（EXD）的详细方案，以便将草药煎剂应用于实验研究。文中描述了适合动物的剂量计算方法，以及二仙汤的四个主要步骤：浸泡、水煎煮、过滤和浓缩。此外，还介绍了将含血清的二仙汤引入大鼠体内作为体外验证手段的方法。在此，给大鼠口服二仙汤三天。然后采集血样，灭活、离心并过滤。用培养基稀释后的血清可用于体外处理细胞或组织。例如，二仙汤被应用于体内和体外研究，并证明其可增强成骨作用。该方案可作为草药制备和应用的参考。
