Nian Hua, Qin Lu-Ping, Zhang Qiao-Yan, Zheng Han-Chen, Yu Yuan, Huang Bao-Kang
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Guohe Road 325, Shanghai 200433, PR China.
J Ethnopharmacol. 2006 Nov 3;108(1):96-102. doi: 10.1016/j.jep.2006.04.020. Epub 2006 May 2.
The antiosteoporotic effect of a herbal formula, Er-Xian Decoction (EXD), in ovariectomized (OVX) rats model of osteoporosis was investigated. The rats were divided into Sham and OVX groups. The OVX rats were further sub-divided into four groups administered orally with water, nylestriol (1 mg/kg, weekly) or EXD (300, 600 mg/kg, daily) for 12 weeks. In OVX rats, the increases of body weight, serum BGP and ALP were significantly decreased by EXD treatment. In OVX rats, atrophy of uterus and descent of BMD were suppressed by treatment with EXD and nylestriol. In addition, EXD completely corrected the decreased concentration of calcium, phosphorus, and estradiol in serum observed in OVX rats. EXD also significantly increased biomechanical strength comparable to the Sham group. This was also confirmed by histological results that showed its protective action. The findings assessed on the basis of biochemical, bone mineral density, biomechanical, and histopathological parameters strongly suggested that EXD had a definite antiosteoporotic effect, which is similar to estrogen.
研究了中药二仙汤（EXD）对去卵巢（OVX）骨质疏松大鼠模型的抗骨质疏松作用。将大鼠分为假手术组和去卵巢组。去卵巢大鼠进一步分为四组，分别口服水、尼尔雌醇（1mg/kg，每周一次）或二仙汤（300、600mg/kg，每日一次），持续12周。在去卵巢大鼠中，二仙汤治疗可显著降低体重、血清骨钙素（BGP）和碱性磷酸酶（ALP）的升高。在去卵巢大鼠中，二仙汤和尼尔雌醇治疗可抑制子宫萎缩和骨密度下降。此外，二仙汤完全纠正了去卵巢大鼠血清中钙、磷和雌二醇浓度的降低。二仙汤还显著提高了生物力学强度，与假手术组相当。组织学结果也证实了其保护作用，进一步证实了这一点。基于生化、骨密度、生物力学和组织病理学参数的研究结果强烈表明，二仙汤具有明确的抗骨质疏松作用，类似于雌激素。
