Belden Sarah E, Uppalapati Chandana K, Pascual Agnes S, Montgomery McKale R, Leyva Kathryn J, Hull Elizabeth E, Averitte Richard L
Affiliated Dermatology & Affiliated Laboratories, Midwestern University Osteopathic Postdoctoral Training Institute, Midwestern University.
Department of Microbiology & Immunology, Arizona College of Osteopathic Medicine, Midwestern University.
J Vis Exp. 2017 May 29(123):55583. doi: 10.3791/55583.
The incidence of skin cancer (e.g., squamous cell carcinoma, basal cell carcinoma, and melanoma) has been increasing over the past several years. It is expected that there will be a parallel demand for cutaneous tumor samples for biomedical research studies. Tissue availability, however, is limited due the cost of establishing a biorepository and the lack of protocols available for obtaining clinical samples that do not interfere with clinical operations. A protocol was established to collect and process cutaneous tumor and associated blood and saliva samples that has minimal impact on routine clinical procedures on the date of a Mohs surgery. Tumor samples are collected and processed from patients undergoing their first layer of Mohs surgery for biopsy-proven cutaneous malignancies by the Mohs histotechnologist. Adjacent normal tissue is collected at the time of surgical closure. Additional samples that may be collected are whole-blood and buccal swabs. By utilizing tissue samples that are normally discarded, a biorepository was generated that offers several key advantages by being based in the clinic versus the laboratory setting. These include a wide range of collected samples; access to de-identified patient records, including pathology reports; and, for the typical donor, access to additional samples during follow-up visits.
在过去几年中，皮肤癌（如鳞状细胞癌、基底细胞癌和黑色素瘤）的发病率一直在上升。预计生物医学研究对皮肤肿瘤样本的需求也会相应增加。然而，由于建立生物样本库的成本以及缺乏不干扰临床操作的获取临床样本的方案，组织的可获得性受到限制。我们制定了一项方案，用于收集和处理皮肤肿瘤以及相关的血液和唾液样本，该方案在莫氏手术当天对常规临床程序的影响最小。莫氏组织技术人员从接受第一层莫氏手术以活检证实为皮肤恶性肿瘤的患者中收集和处理肿瘤样本。手术缝合时收集相邻的正常组织。可能收集的其他样本是全血和颊拭子。通过利用通常被丢弃的组织样本，建立了一个生物样本库，与实验室环境相比，该样本库设在临床具有几个关键优势。这些优势包括收集的样本种类广泛；能够获取去识别化的患者记录，包括病理报告；而且，对于典型的捐赠者来说，在随访期间能够获取额外的样本。
