Reyes E, Murray R W
Neurobehav Toxicol Teratol. 1985 Mar-Apr;7(2):177-80.
Changes in gamma-glutamyl transpeptidase (gamma-GTP) activity have been shown to occur concomitantly with ethanol use. Alterations in gamma-GTP isozymal forms have also been demonstrated to accompany various developmental stages, diseases, and drug exposures. In view of these properties, electrophoretic examination of gamma-GTP isozymal forms was performed on sera from neonatal rats exposed to ethanol during gestation. The purpose of this study was to see whether or not a form of the enzyme specific for prenatal ethanol exposure could be demonstrated. Pregnant Wistar rats were pair-fed on diets containing either ethanol (1, 4, 5, or 6.7% v/v) or diets without ethanol which were isocalorically equivalent. Sera collected from one day old neonates were separated into gamma-GTP fractions using polyacrylamide gradient gel electrophoresis. The zymograms were specifically stained for gamma-GTP activity and desitometrically scanned. All alcohol groups had 4 bands of gamma-GTP activity shared in common with the control groups. However, in addition to these bands the sera from the neonates exposed to ethanol in utero were found to have a unique band of gamma-GTP activity. This unique band was present in all zymograms of sera from ethanol-exposed neonates and absent in all control neonates. The possibility therefore exists that gamma-GTP serum isozymes may be useful as a diagnostic index available for the assessment of Fetal alcohol exposure in man.
γ-谷氨酰转肽酶（γ-GTP）活性的变化已被证明与乙醇的使用同时发生。γ-GTP同工酶形式的改变也已被证明伴随着各种发育阶段、疾病和药物暴露。鉴于这些特性，对孕期暴露于乙醇的新生大鼠血清进行了γ-GTP同工酶形式的电泳检查。本研究的目的是观察是否能证明存在一种对产前乙醇暴露具有特异性的酶形式。将怀孕的Wistar大鼠成对饲养在含有乙醇（1%、4%、5%或6.7%体积/体积）的饮食或不含乙醇但热量相等的饮食中。从出生一天的新生儿采集的血清通过聚丙烯酰胺梯度凝胶电泳分离成γ-GTP组分。酶谱用γ-GTP活性进行特异性染色并进行光密度扫描。所有乙醇组都有4条γ-GTP活性带，与对照组相同。然而，除了这些条带外，发现子宫内暴露于乙醇的新生儿血清还有一条独特的γ-GTP活性带。这条独特的带存在于乙醇暴露新生儿血清的所有酶谱中，而在所有对照新生儿中不存在。因此，γ-GTP血清同工酶有可能作为一种诊断指标，用于评估人类胎儿酒精暴露情况。
