Valkó Anna, Biksi Imre, Cságola Attila, Tuboly Tamás, Kiss Krisztián, Ursu Krisztina, Dán Ádám
Department of Microbiology and Infectious Diseases, University of Veterinary Medicine Budapest , Hungária krt. 23-25, H-1143 Budapest , Hungary.
Department and Clinic for Production Animals, University of Veterinary Medicine Budapest , Üllő, Dóra major , Hungary.
Acta Vet Hung. 2017 Jun;65(2):253-261. doi: 10.1556/004.2017.025.
Porcine epidemic diarrhoea virus (PEDV) can cause a severe enteric disease affecting pigs of all ages. In January 2016, diarrhoea with occasional vomiting was observed in a small pig farm in Hungary. All animals became affected, while mortality (of up to 30%) was only seen in piglets. Samples from different age groups and the carcass of a piglet were examined by various methods including pathology, bacteriology and molecular biology. PEDV was confirmed by PCR and its whole genome sequence was determined. The sequence PEDV HUN/5031/2016 showed high identity with recently reported European viruses. Differences were found mostly in the S gene, where recombination was detected with a newly identified and already recombinant swine enteric coronavirus (Se-CoV) from Italy. The present report describes the first porcine epidemic diarrhoea outbreak in Hungary after many years and gives an insight into the genetics of the Hungarian PEDV.
猪流行性腹泻病毒（PEDV）可引发一种严重的肠道疾病，影响所有年龄段的猪。2016年1月，匈牙利的一个小型养猪场出现了腹泻并伴有偶尔呕吐的情况。所有动物均受到影响，然而仅在仔猪中出现了高达30%的死亡率。通过病理学、细菌学和分子生物学等多种方法，对来自不同年龄组的样本以及一头仔猪的尸体进行了检测。通过PCR确认了PEDV，并测定了其全基因组序列。序列PEDV HUN/5031/2016与最近报道的欧洲病毒具有高度同源性。差异主要存在于S基因中，在该基因中检测到与来自意大利的一种新鉴定且已发生重组的猪肠道冠状病毒（Se-CoV）发生了重组。本报告描述了匈牙利多年来首次猪流行性腹泻疫情，并深入分析了匈牙利PEDV的遗传学特征。
