Jiang Lu-Yong, Yu Xiao, Pang Qing-Jiang
Department of Orthopedics, Ningbo No. 2 Hospital, Ningbo, Zhejiang Province, China.
J Int Med Res. 2017 Aug;45(4):1324-1331. doi: 10.1177/0300060517707076. Epub 2017 Jun 12.
Objective To elucidate the effects of recombinant human erythropoietin (rHuEPO) on steroid-induced osteonecrosis of the femoral head in rats. Methods Twenty-four adult Wistar rats were randomly divided into three groups of eight rats each. The rats in the positive control group were injected with dexamethasone at 1 mg/kg twice a week for 5 weeks. The rats in the negative control group were injected with sodium chloride alone. The rats in the experimental group were injected with dexamethasone at 1 mg/kg twice a week for 5 weeks and rHuEPO (500 u/d/kg) daily for 5 weeks. The femoral head on one side was examined by hematoxylin and eosin staining, and that on the other side was examined by CD31 staining of the capillaries. Results Hematoxylin and eosin staining in the positive control group showed that the bony trabeculae had become obviously narrow and sparse with discontinuity of the integrity. The integrity of the trabeculae was better in the experimental group than positive control group. The CD31 expression was lower in the positive control group than in the other two groups. Conclusion rHuEPO can effectively prevent osteocyte apoptosis, delaying or decreasing osteonecrosis of the femoral head.
目的 阐明重组人促红细胞生成素（rHuEPO）对大鼠激素性股骨头坏死的影响。方法 将24只成年Wistar大鼠随机分为三组，每组8只。阳性对照组大鼠每周两次注射地塞米松，剂量为1mg/kg，共5周。阴性对照组大鼠仅注射氯化钠。实验组大鼠每周两次注射地塞米松，剂量为1mg/kg，共5周，同时每天注射rHuEPO（500u/d/kg），共5周。一侧股骨头进行苏木精-伊红染色检查，另一侧股骨头进行毛细血管CD31染色检查。结果 阳性对照组苏木精-伊红染色显示骨小梁明显变窄、稀疏，完整性中断。实验组骨小梁完整性优于阳性对照组。阳性对照组CD31表达低于其他两组。结论 rHuEPO可有效预防骨细胞凋亡，延缓或减少股骨头坏死。
