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土耳其安卡拉犬细粒棘球绦虫感染的粪便诊断

Coprodiagnosis of Echinococcus granulosus infection in dogs from Ankara, Turkey.

作者信息

Öge Hatice, Öge Semih, Gönenç Bahadır, Sarımehmetoğlu Oğuz, Özbakış Gökben

机构信息

Ankara University, Faculty of Veterinary Medicine, Department of Parasitology, 06110 Ankara, Turkey.

Ankara University, Faculty of Veterinary Medicine, Department of Parasitology, 06110 Ankara, Turkey.

出版信息

Vet Parasitol. 2017 Aug 15;242:44-46. doi: 10.1016/j.vetpar.2017.05.016. Epub 2017 May 22.

Abstract

The present study were undertaken to compare two isolation techniques (centrifugal flotation and sedimentation) for recovering taeniid eggs from faecal samples, to identify E. granulosus DNA from taeniid eggs by PCR, and to determine the prevalence of E. granulosus in dogs in villages. Faecal samples were collected from 100 dogs in Ankara province. Taenia spp. eggs were found in 27% of dogs faeces. Echinococcus granulosus-specific PCR was obtained in 14 (51.85%) of the taeniid eggs-positive samples. As well as finding Taenia eggs in dogs' faeces, we also found eggs of some helminthic parasites; such as Dipylidium caninum, Toxocara canis, Toxascaris leonina, Trichuris sp., Capillaria sp., Filaroides sp., Dioctophyme renale, Linguatula serrata, hookworm, Dicrocoelium sp., Fasciola sp. and Ascaridia galli. Significantly, more dogs excreting taeniid eggs were diagnosed with the sedimentation method (n=27) as compared to the flotation method (n=10).

摘要

本研究旨在比较两种从粪便样本中回收带绦虫卵的分离技术(离心浮选法和沉淀法),通过聚合酶链反应(PCR)从带绦虫卵中鉴定细粒棘球绦虫DNA,并确定农村地区犬类细粒棘球绦虫的患病率。从安卡拉省的100只狗身上采集了粪便样本。在27%的狗粪便中发现了带绦虫属的卵。在14份(51.85%)带绦虫卵阳性样本中获得了细粒棘球绦虫特异性PCR结果。除了在狗粪便中发现带绦虫卵外,我们还发现了一些蠕虫寄生虫的卵,如犬复孔绦虫、犬弓首蛔虫、狮弓蛔虫、毛首线虫属、毛细线虫属、类丝虫属、肾膨结线虫、锯齿舌形虫、钩虫、双腔吸虫属、片形吸虫属和鸡蛔虫。值得注意的是,与浮选法(n = 10)相比,采用沉淀法诊断出排泄带绦虫卵的狗更多(n = 27)。

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