Orsten Serra, Boufana Belgees, Ciftci Turkmen, Akinci Devrim, Karaagaoglu Ergun, Ozkuyumcu Cumhur, Casulli Adriano, Akhan Okan
School of Health Services, Hacettepe University, Ankara, Turkey.
Department of Infectious Diseases, WHO Collaborating Centre for the Epidemiology, Detection and Control of CE and AE, European Union Reference Laboratory for Parasites, Istituto Superiore di Sanitá, Rome, Italy.
Parasitol Res. 2018 Apr;117(4):1257-1263. doi: 10.1007/s00436-018-5807-9. Epub 2018 Feb 23.
Cystic echinococcosis caused by the larval stages of Echinococcus granulosus sensu lato s.l is endemic in Turkey with a high public health impact particularly in rural areas. The aim of this study was to investigate the genetic variation and population structure of E. granulosus s.s using metacestode isolates removed from surgically confirmed patients originating from several regions in Turkey and to investigate the occurrence of autochthonous transmission. Using DNA extracted from a total of 46 human-derived CE isolates, we successfully analysed an 827-bp fragment within the cox1 mitochondrial gene and confirmed the causative agent of human cystic echinococcosis in patients included in this study to be Echinococcus granulosus s.s (G1 and G3 genotypes). The haplotype parsimony network consisted of 28 haplotypes arranged within three main clusters and the neutrality indices were both negative and significant indicating negative selection or population expansion. The assessment carried out in this study using GenBank nucleotide sequence data from Turkey for sheep and cattle hosts demonstrated the importance of autochthonous transmission with sheep, cattle and humans harbouring the same haplotypes. Further studies are required to investigate the biological significance, if any, of E. granulosus s.s haplotypes and the genetic variability of CE from human patients using longer nucleotide sequences and a larger sample set.
由细粒棘球绦虫广义种幼虫阶段引起的囊型包虫病在土耳其呈地方性流行，对公共卫生影响极大，尤其是在农村地区。本研究旨在利用从土耳其多个地区经手术确诊患者体内取出的棘球蚴分离株，调查细粒棘球绦虫狭义种的遗传变异和种群结构，并调查本地传播的发生情况。我们从总共46株人源囊型包虫病分离株中提取DNA，成功分析了细胞色素c氧化酶亚基1（cox1）线粒体基因内一个827 bp的片段，并确认本研究中患者的人囊型包虫病病原体为细粒棘球绦虫狭义种（G1和G3基因型）。单倍型简约网络由28个单倍型组成，排列在三个主要簇中，中性指数均为负且显著，表明存在负选择或种群扩张。本研究利用来自土耳其绵羊和牛宿主的GenBank核苷酸序列数据进行的评估表明，本地传播很重要，绵羊、牛和人类携带相同的单倍型。需要进一步研究以调查细粒棘球绦虫狭义种单倍型的生物学意义（若有），以及使用更长的核苷酸序列和更大的样本集研究来自人类患者的囊型包虫病的遗传变异性。
