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[用聚合酶链反应测定艾登地区犬中细粒棘球绦虫的患病率]

[Prevalence of Echinococcus granulosus determined with polymerase chain reaction in dogs in Aydın district].

作者信息

Kuru Buket Boğa, Aypak Süleyman, Aysul Nuran

机构信息

Adnan Menderes Üniversitesi Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Aydın, Turkey.

出版信息

Turkiye Parazitol Derg. 2013;37(2):78-83. doi: 10.5152/tpd.2013.20.

Abstract

OBJECTIVE

In this study, it was aimed to determine the prevalence of E. granulosus in the Aydın district.

METHODS

Clean and fresh faeces samples were collected off the ground from owned dogs (n=100; 25 female, 75 male) with the range of 6-16 months old. These faeces samples were obtained in 13 villages from five different locations (Centre, Söke, Çine, Bozdoğan and Kuyucak) of the Aydın district. The prevalence of E. granulosus was investigated with PCR and prior to applications of molecular techniques, faeces samples were investigated with macroscopic and floatation methods

RESULTS

E. granulosus was determined in only one dog (1%; 1/100) and Toxocara canis (11%), Ancylostoma caninum (%3), Taenia spp. (2%) and Capillaria spp. (1%) eggs were also identified.

CONCLUSION

Current prevalence (1%) of E. granulosus in this study is lower than the other studies conducted in different parts of Turkey. Determination of E. granulosus with PCR in faeces without determining any parasites with either macroscopic or microscopic examinations could indicate the potential risk of dog faeces for animal owners, veterinarians and parasitology laboratory technicians.

摘要

目的

本研究旨在确定艾登地区细粒棘球绦虫的流行情况。

方法

从6至16月龄的家养犬(n = 100;25只雌性,75只雄性)的地面上采集清洁新鲜的粪便样本。这些粪便样本取自艾登地区五个不同地点(中心、索克、Çine、博兹多安和库尤贾克)的13个村庄。采用聚合酶链反应(PCR)研究细粒棘球绦虫的流行情况,在应用分子技术之前,先用宏观和漂浮法对粪便样本进行检查。

结果

仅在一只犬(1%;1/100)中检测到细粒棘球绦虫,还鉴定出犬弓首蛔虫(11%)、犬钩虫(3%)、带绦虫属(2%)和毛细线虫属(1%)的虫卵。

结论

本研究中细粒棘球绦虫的当前流行率(1%)低于在土耳其不同地区进行的其他研究。在未通过宏观或微观检查确定任何寄生虫的情况下,用PCR在粪便中检测到细粒棘球绦虫可能表明犬粪便对动物主人、兽医和寄生虫学实验室技术人员存在潜在风险。

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