Zhang Xi, Duan Jiang Yang, Shi Ya Li, Jiang Peng, Zeng De Jun, Wang Zhong Quan, Cui Jing
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China.
Department of Parasitology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China.
Mol Phylogenet Evol. 2017 Dec;117:75-82. doi: 10.1016/j.ympev.2017.06.003. Epub 2017 Jun 9.
The larva of Spirometra erinaceieuropaei can parasitize humans, causing a serious parasitic zoonosis known as sparganosis. Although it is medically important, our knowledge about the phylogenetic position of S. erinaceieuropaei and its evolutionary history is fragmentary. In this study, complete mitochondrial (mt) genomes of 4 geographically distinct isolates of S. erinaceieuropaei spargana collected from 4 frog hosts (Hylarana guentheri, Rana nigromaculata, R. rugulosa, R. temporaria) were characterized using an Illumina sequencing platform. In addition, all available mt genomes of Cestoda in GenBank were included to reconstruct the phylogeny and to explore the evolutionary history of these tapeworms. The genome features of S. erinaceieuropaei contained 12 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions. Nucleotide sequences of mtDNA from different frog hosts were similar. Three genes, cox1, cytb and nad4, had high levels of nucleotide diversity. Phylogenetic analyses supported the sibling relationship between Bothriocephalidae and Diphyllobothriidae. Molecular dating analysis indicated that the divergence between Diphyllobothrium and Diplogonoporus started in the late Miocene. The mt genomes of S. erinaceieuropaei will serve as a useful dataset for studying the genetics and systematics of the species of Spirometra genus in particular and diphyllobothriid tapeworms in general.
曼氏迭宫绦虫的幼虫可寄生于人体，引发一种严重的寄生虫人畜共患病，称为裂头蚴病。尽管其具有医学重要性，但我们对曼氏迭宫绦虫的系统发育位置及其进化历史的了解仍不完整。在本研究中，利用Illumina测序平台对从4种蛙类宿主（泽蛙、黑斑侧褶蛙、虎纹蛙、欧洲林蛙）采集的4个地理上不同的曼氏迭宫绦虫裂头蚴分离株的完整线粒体（mt）基因组进行了特征分析。此外，还纳入了GenBank中所有可用的绦虫mt基因组，以重建系统发育并探索这些绦虫的进化历史。曼氏迭宫绦虫的基因组特征包括12个蛋白质编码基因（PCGs）、22个转运RNA基因、2个核糖体RNA基因和2个非编码区。来自不同蛙类宿主的mtDNA核苷酸序列相似。cox1、cytb和nad4这三个基因具有较高的核苷酸多样性水平。系统发育分析支持了裂头科和双叶槽科之间的姐妹关系。分子年代分析表明，双叶槽属和双孔属之间的分化始于中新世晚期。曼氏迭宫绦虫的mt基因组将作为一个有用的数据集，尤其用于研究迭宫属物种以及一般双叶槽科绦虫的遗传学和系统学。
