College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, China.
Int J Biol Sci. 2012;8(5):640-9. doi: 10.7150/ijbs.4096. Epub 2012 Apr 27.
Sparganosis, caused by the plerocercoid larvae of members of the genus Spirometra, can cause significant public health problem and considerable economic losses. In the present study, the complete mitochondrial DNA (mtDNA) sequence of Spirometra erinaceieuropaei from China was determined, characterized and compared with that of S. erinaceieuropaei from Japan. The gene arrangement in the mt genome sequences of S. erinaceieuropaei from China and Japan is identical. The identity of the mt genomes was 99.1% between S. erinaceieuropaei from China and Japan, and the complete mtDNA sequence of S. erinaceieuropaei from China is slightly shorter (2 bp) than that from Japan. Phylogenetic analysis of S. erinaceieuropaei with other representative cestodes using two different computational algorithms [Bayesian inference (BI) and maximum likelihood (ML)] based on concatenated amino acid sequences of 12 protein-coding genes, revealed that S. erinaceieuropaei is closely related to Diphyllobothrium spp., supporting classification based on morphological features. The present study determined the complete mtDNA sequences of S. erinaceieuropaei from China that provides novel genetic markers for studying the population genetics and molecular epidemiology of S. erinaceieuropaei in humans and animals.
裂头蚴病是由裂头蚴幼虫引起的，可导致严重的公共卫生问题和巨大的经济损失。本研究测定了来自中国的曼氏迭宫绦虫的完整线粒体 DNA（mtDNA）序列，并对其进行了特征描述和与来自日本的曼氏迭宫绦虫进行了比较。中国和日本的曼氏迭宫绦虫 mt 基因组序列的基因排列是相同的。来自中国和日本的曼氏迭宫绦虫的 mt 基因组的同一性为 99.1%，来自中国的曼氏迭宫绦虫的完整 mtDNA 序列比来自日本的短（2bp）。基于 12 个蛋白编码基因的氨基酸序列的两种不同计算算法[贝叶斯推断（BI）和最大似然（ML）]对曼氏迭宫绦虫与其他代表性绦虫的系统发育分析表明，曼氏迭宫绦虫与双槽绦虫属密切相关，支持基于形态特征的分类。本研究确定了来自中国的曼氏迭宫绦虫的完整 mtDNA 序列，为研究人和动物中曼氏迭宫绦虫的种群遗传学和分子流行病学提供了新的遗传标记。
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