State Key Laboratory of Food Science and Technology, Nanchang University , Nanchang 330047, China.
Institute of Life Science & College of Life Sciences, Nanchang University , Nanchang 330031, China.
J Agric Food Chem. 2017 Jul 5;65(26):5306-5315. doi: 10.1021/acs.jafc.7b01763. Epub 2017 Jun 26.
Our previous study has demonstrated that Ganoderma atrum polysaccharide (PSG-1) has immunomodulatory activity on spleen lymphocytes. However, how PSG-1 exerts its effect on purified lymphocytes is still obscure. Thus, this study aimed to investigate the immunomodulatory activity of PSG-1 on purified T lymphocytes and further elucidate the underlying mechanism based on RNA sequencing (RNA-seq). Our results showed that PSG-1 promoted T lymphocytes proliferation and increased the production of IL-2, IFN-γ, and IL-12. Meanwhile, RNA-seq analysis found 394 differentially expressed genes. KEGG pathway analysis identified 20 significant canonical pathways and seven biological functions. Furthermore, PSG-1 elevated intracellular Ca concentration and calcineurin (CaN) activity and raised the p-ERK, p-JNK, and p-p38 expression levels. T lymphocytes proliferation and the production of IL-2, IFN-γ, and IL-12 were decreased by the inhibitors of calcium channel and mitogen-activated protein kinases (MAPKs). These results indicated that PSG-1 possesses immunomodulatory activity on purified T lymphocytes, in which Ca/CaN and MAPK pathways play essential roles.
我们之前的研究表明，黑灵芝多糖（PSG-1）对脾淋巴细胞具有免疫调节活性。然而，PSG-1 如何对纯化的淋巴细胞发挥作用仍不清楚。因此，本研究旨在探讨 PSG-1 对纯化 T 淋巴细胞的免疫调节活性，并基于 RNA 测序（RNA-seq）进一步阐明其潜在机制。我们的结果表明，PSG-1 可促进 T 淋巴细胞增殖，并增加白细胞介素-2（IL-2）、干扰素-γ（IFN-γ）和白细胞介素-12（IL-12）的产生。同时，RNA-seq 分析发现 394 个差异表达基因。KEGG 通路分析确定了 20 个显著的经典通路和 7 个生物学功能。此外，PSG-1 增加了细胞内钙浓度和钙调神经磷酸酶（CaN）活性，并提高了 ERK、JNK 和 p38 的磷酸化水平。钙通道和丝裂原活化蛋白激酶（MAPKs）抑制剂降低了 T 淋巴细胞的增殖和白细胞介素-2、干扰素-γ和白细胞介素-12 的产生。这些结果表明，PSG-1 对纯化的 T 淋巴细胞具有免疫调节活性，其中 Ca/CaN 和 MAPK 途径发挥着重要作用。
