Li Qiu, Li Qun, Hao Zhihui, Zheng Xucai, He Wei
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau Macau SAR China.
Department of Immunology, School of Basic Medical Sciences, Anhui Medical University Hefei China
RSC Adv. 2018 Jul 23;8(46):26371-26376. doi: 10.1039/c8ra02923g. eCollection 2018 Jul 19.
We report a novel water-soluble polysaccharide from , namely, PJ-1 with anti-inflammatory activity. We first obtained a polysaccharide from this herb by eluting with water. After fully characterizing PJ-1 with a series of chromatographic technologies, we evaluated its biological activity by stimulating Raw 264.7 cells to express inflammatory cytokines and found that PJ-1 effectively enhanced the production of anti-inflammatory factors such as IL-10. We further identified its anti-inflammatory effect in a mouse model of sepsis induced by a lipopolysaccharide. The treatment of PJ-1 evidently suppressed the secretion of major inflammatory cytokines and increased the survival rate of mice. Particularly, we verified the STAT3 signal pathway responsible for the anti-inflammatory activity. Thus, PJ-1, which has remarkable anti-inflammatory effect and mechanism of action, may be a promising candidate as an anti-inflammatory agent.
我们报道了一种从[草药名称未给出]中提取的具有抗炎活性的新型水溶性多糖，即PJ - 1。我们首先通过用水洗脱从这种草药中获得了一种多糖。在用一系列色谱技术对PJ - 1进行全面表征后，我们通过刺激Raw 264.7细胞表达炎性细胞因子来评估其生物活性，发现PJ - 1有效地增强了抗炎因子如IL - 10的产生。我们进一步在脂多糖诱导的脓毒症小鼠模型中确定了其抗炎作用。PJ - 1处理明显抑制了主要炎性细胞因子的分泌并提高了小鼠的存活率。特别地，我们验证了负责抗炎活性的STAT3信号通路。因此，具有显著抗炎作用和作用机制的PJ - 1可能是一种有前途的抗炎剂候选物。
