Spakova Timea, Amrichova Judita, Plsikova Jana, Harvanova Denisa, Hornak Slavomir, Ledecky Valent, Rosocha Jan
1 Associated Tissue Bank, Faculty of Medicine, P. J. Safarik University and L. Pasteur University Hospital, Kosice, Slovakia.
2 Clinic of Small Animals, University of Veterinary Medicine and Pharmacy, Kosice, Slovakia.
Cartilage. 2018 Oct;9(4):410-416. doi: 10.1177/1947603517713816. Epub 2017 Jun 13.
Objective This study aimed to compare microfracture and application of adipose-derived stem cells (ADSCs) by local adherent technique enhanced by platelet-rich plasma (PRP) to provide a new approach for the repair of cartilage defect. Design Full-thickness cylindrical defects were created in the medial femoral condyle in 9 New Zealand White rabbits (5 months old, 4.65 ± 0.20 kg). Two groups of rabbits ( n = 3) were either treated with ADSCs (Group 1) or the microfracture technique (Group 2) following intraarticular injection of PRP 3 times in weekly intervals. Rabbits in control group ( n = 3) remained untreated. The outcome was assessed macroscopically, histologically, and immunohistochemically. Results At the end of week 12, Group 1 showed better defect filling compared with Group 2. Specimens treated with the combination of ADSCs and PRP exhibited significant differences from the other groups in all criteria of International Cartilage Repair Society macroscopic scoring system. Conclusions Intraarticular injection of autologous PRP in combination with transplantation of autologous ADSCs by local adherent technique enhances the quality of cartilage defect repair with better results in comparison with microfracture surgery in a rabbit model.
目的 本研究旨在比较微骨折术与通过富含血小板血浆（PRP）增强的局部贴附技术应用脂肪源性干细胞（ADSCs），为软骨缺损修复提供新方法。设计 在9只新西兰白兔（5月龄，4.65±0.20 kg）的股骨内侧髁制造全层圆柱形缺损。两组兔子（n = 3）分别在每周关节腔内注射3次PRP后，接受ADSCs治疗（第1组）或微骨折技术治疗（第2组）。对照组兔子（n = 3）不做处理。通过宏观、组织学和免疫组织化学方法评估结果。结果 在第12周结束时，第1组缺损填充情况优于第2组。ADSCs与PRP联合处理的标本在国际软骨修复协会宏观评分系统的所有标准方面与其他组均存在显著差异。结论 在兔模型中，关节腔内注射自体PRP并结合通过局部贴附技术移植自体ADSCs可提高软骨缺损修复质量，与微骨折手术相比效果更好。
