Deng Zhantao, Jin Jiewen, Wang Shuai, Qi Fangjie, Chen Xuepan, Liu Chang, Li Yanbing, Ma Yuanchen, Lyu Fengjuan, Zheng Qiujian
Department of Orthopedics, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
Department of Endocrinology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Ann Transl Med. 2020 Dec;8(23):1598. doi: 10.21037/atm-20-2342.
Stem cell-based therapy is a promising treatment for cartilage defects due to the pluripotency, abundant sources and low immunogenicity of stem cells. Hydrogels are a promising class of biomaterials for cartilage engineering and are characterized by bioactivity, degradability and elasticity as well as provide water content and mechanical support. The combination of stem cells and hydrogels opens new possibilities for cartilage tissue engineering. However, the selection of suitable types of stem cells and hydrogels is difficult. Currently, various types of stem cells, such as embryonic stem cells (ESCs), mesenchymal stem cells (MSCs), induced pluripotent stem cells (iPSCs), and peripheral blood mononuclear cells (PBMSCs), and various types of hydrogels, including natural polymers, chemically modified natural polymers and synthetic polymers, have been explored based on their potential for cartilage tissue engineering. These materials are used independently or in combination; however, there is no clear understanding of their merits and disadvantages with regard to their suitability for cartilage repair. In this article, we aim to review recent progress in the use of stem cell-hydrogel hybrid constructs for cartilage tissue engineering. We focus on the effects of stem cell types and hydrogel types on efficient chondrogenesis from cellular, preclinical and clinical perspectives. We compare and analyze the advantages and disadvantages of these cells and hydrogels with the hope of increasing discussion of their suitability for cartilage repair and present our perspective on their use for the improvement of physical and biological properties for cartilage tissue engineering.
基于干细胞的疗法是一种很有前景的治疗软骨缺损的方法，因为干细胞具有多能性、来源丰富且免疫原性低。水凝胶是一类很有前景的用于软骨工程的生物材料，其特点是具有生物活性、可降解性和弹性，还能提供含水量和机械支撑。干细胞与水凝胶的结合为软骨组织工程开辟了新的可能性。然而，选择合适类型的干细胞和水凝胶很困难。目前，基于其在软骨组织工程中的潜力，人们已经探索了各种类型的干细胞，如胚胎干细胞（ESC）、间充质干细胞（MSC）、诱导多能干细胞（iPSC）和外周血单个核细胞（PBMSC），以及各种类型的水凝胶，包括天然聚合物、化学修饰的天然聚合物和合成聚合物。这些材料单独使用或联合使用；然而，对于它们在软骨修复适用性方面的优缺点尚无清晰认识。在本文中，我们旨在综述用于软骨组织工程的干细胞 - 水凝胶杂交构建体的最新进展。我们从细胞、临床前和临床角度关注干细胞类型和水凝胶类型对有效软骨形成的影响。我们比较并分析这些细胞和水凝胶的优缺点，希望增加对它们在软骨修复适用性方面的讨论，并阐述我们对其用于改善软骨组织工程物理和生物学特性的看法。
