豚鼠新皮质中神经肽Y样免疫反应性的亚细胞分布

Subcellular distribution of neuropeptide Y-like immunoreactivity in guinea pig neocortex.

作者信息

de Quidt M E, Richardson P J, Emson P C

出版信息

Brain Res. 1985 Jun 3;335(2):354-9. doi: 10.1016/0006-8993(85)90493-7.

Abstract

Neuropeptide Y-like immunoreactivity (NPY-LI) was enriched in synaptosomal fractions of neocortex, which on lysis yielded vesicle-rich fractions. The distribution of NPY-LI on a sucrose density gradient was similar to that of somatostatin, with a concentration in heavy vesicles. The peptides were not found in light vesicles in contrast to the distribution of noradrenaline. Both homogenate and vesicular NPY-LI coeluted with synthetic NPY on reverse-phase HPLC.

摘要

神经肽Y样免疫反应性（NPY-LI）在新皮质的突触体组分中富集，该组分裂解后产生富含囊泡的组分。NPY-LI在蔗糖密度梯度上的分布与生长抑素相似，在重囊泡中有浓度峰值。与去甲肾上腺素的分布不同，在轻囊泡中未发现这些肽。在反相高效液相色谱法中，匀浆和囊泡中的NPY-LI均与合成NPY共洗脱。