Beal M F, Mazurek M F, Lorenz L J, Chattha G K, Ellison D W, Martin J B
Neurosci Lett. 1986 Feb 14;64(1):69-74. doi: 10.1016/0304-3940(86)90665-8.
A rabbit antiserum to neuropeptide Y (NPY) was used to develop a radioimmunoassay for measuring NPY in brain tissue. By high-performance liquid chromatography, two peaks of immunoreactivity were detected in human postmortem cortex. A minor peak was seen at the void volume, while the majority of immunoreactivity comigrated with synthetic NPY standards. Using the Spokes-Koch rat model simulating human autopsy conditions, it was shown that cortical, hippocampal and striatal concentrations of NPY-like immunoreactivity are stable for up to 24 h.
一种针对神经肽Y（NPY）的兔抗血清被用于开发一种放射免疫分析法，以测量脑组织中的NPY。通过高效液相色谱法，在人类死后皮层中检测到两个免疫反应峰。在空体积处可见一个较小的峰，而大部分免疫反应性与合成NPY标准品同时洗脱。使用模拟人类尸检条件的Spokes-Koch大鼠模型，结果表明，皮层、海马和纹状体中NPY样免疫反应性的浓度在长达24小时内是稳定的。
