Department of Chemistry, Sungkyunkwan University, 2066 Seobu-Ro, Jangan-Gu, Suwon, Gyeonggi-Do 440-746, Republic of Korea.
Bio-Medical IT Convergence Research Department, Electronics and Telecommunications Research Institute (ETRI), Daejeon 305-700, Republic of Korea.
Biosens Bioelectron. 2017 Nov 15;97:273-277. doi: 10.1016/j.bios.2017.06.012. Epub 2017 Jun 8.
Nanogap biosensor shows a distinct conduction change upon sandwich-type immobilization of gold nanoparticle probes onto the gap region in the presence of target biomolecules. Although this large conductance change could be advantageous in distinguishing signal on or off devices, since the extent of conductance change is quite irregular even at the same analyte concentrations, it fails to extract quantitative information from its level of conductance change. In other words, the conductance change of a single device does not reflect the concentration of the target molecule. In this study, we introduce an alternative approach of interpreting the concentration of target molecules using digital domain analysis of integrated nanogap devices, where the fraction of signal-on-devices, or on-device-percentage (ODP), was translated into the concentration of the target molecule. The ODP was found to be closely related to the number density of the immobilized probes and, therefore, to be an excellent measure of the analyte concentration, which was demonstrated in the immuno-selective detection and quantification of influenza A hemagglutinin and prostate specific antigen.
纳诺间隙生物传感器在目标生物分子存在的情况下，通过将金纳米颗粒探针三明治式固定在间隙区域，显示出明显的传导变化。虽然这种大的传导变化在区分信号开或关设备方面可能是有利的，但由于即使在相同的分析物浓度下，传导变化的程度也相当不规则，因此无法从其传导变化水平中提取定量信息。换句话说，单个设备的传导变化并不反映目标分子的浓度。在这项研究中，我们引入了一种替代方法，即使用集成纳诺间隙设备的数字域分析来解释目标分子的浓度，其中信号开启设备的分数，或设备开启百分比（ODP），被转换为目标分子的浓度。ODP 被发现与固定探针的数密度密切相关，因此是分析物浓度的一个很好的衡量标准，这在流感 A 血凝素和前列腺特异性抗原的免疫选择性检测和定量中得到了证明。
