Pyo Hannah, Lee Cho Yeon, Kim Daehee, Kim Gyuhee, Lee Sangho, Yun Wan Soo
Department of Chemistry, Sungkyunkwan University (SKKU), Suwon 440-746, Korea.
Department of Biological Sciences, Sungkyunkwan University (SKKU), Suwon 440-746, Korea.
Sensors (Basel). 2017 Sep 2;17(9):2012. doi: 10.3390/s17092012.
A simple method of nanoparticle decoration can be used in the detection of pneumococcus. After the pneumococcal bacteria were captured by an antibody (pneumococcal C-polysaccharide (PnC) antibody) between the interdigitated electrodes, the gold nanoparticles conjugated with the PnC antibodies were let to bind onto an outer membrane of the bacteria. Upon successfully dense decoration, the bacteria surface will become conductive owing to the metal nanoparticles, and a distinctive conductance change between the electrodes can be observed. Since this success ratio, or the probability of the conductance change, reflects the concentration of the analyte, a number of repeated measurements can be used in the quantification of the bacteria. In this way, we have successfully detected in the range of 10-10⁸ CFU/mL. The limit of detection in this work is lower than that in the commercial detection kit. We hope that the nanoparticle decoration method will play a role in the facile detection of various bacteria.
一种简单的纳米颗粒修饰方法可用于肺炎球菌的检测。在叉指电极之间，肺炎球菌被抗体（肺炎球菌C多糖（PnC）抗体）捕获后，将与PnC抗体偶联的金纳米颗粒使其结合到细菌的外膜上。成功进行密集修饰后，由于金属纳米颗粒，细菌表面将变得具有导电性，并且可以观察到电极之间独特的电导变化。由于这种成功率或电导变化的概率反映了分析物的浓度，因此可以通过多次重复测量来对细菌进行定量。通过这种方式，我们成功检测到的范围为10 - 10⁸CFU/mL。这项工作中的检测限低于商业检测试剂盒中的检测限。我们希望纳米颗粒修饰方法将在各种细菌的简便检测中发挥作用。
