Sarioglu Omer Faruk, San Keskin Nalan Oya, Celebioglu Asli, Tekinay Turgay, Uyar Tamer
Institute of Materials Science & Nanotechnology, UNAM-National Nanotechnology Research Center Bilkent University, 06800, Bilkent, Ankara, Turkey.
Polatlı Science and Literature Faculty, Biology Department, Gazi University, 06900, Polatlı, Ankara, Turkey; Life Sciences Application and Research Center, Gazi University, 06830, Golbasi, Ankara, Turkey.
Chemosphere. 2017 Oct;184:393-399. doi: 10.1016/j.chemosphere.2017.06.020. Epub 2017 Jun 6.
In this study, preparation and application of novel biocomposite materials for textile dye removal which are produced by immobilization of specific bacteria onto electrospun nanofibrous webs are presented. A textile dye remediating bacterial isolate, Clavibacter michiganensis, was selected for bacterial immobilization, a commercial reactive textile dye, Setazol Blue BRF-X, was selected as the target contaminant, and electrospun polycaprolactone (PCL) and polylactic acid (PLA) nanofibrous polymeric webs were selected for bacterial integration. Bacterial adhesion onto nanofibrous webs was monitored by scanning electron microscopy (SEM) imaging and optical density (OD) measurements were performed for the detached bacteria. After achieving sufficient amounts of immobilized bacteria on electrospun nanofibrous webs, equivalent web samples were utilized for testing the dye removal capabilities. Both bacteria/PCL and bacteria/PLA webs have shown efficient remediation of Setazol Blue BRF-X dye within 48 h at each tested concentration (50, 100 and 200 mg/L), and their removal performances were very similar to the free-bacteria cells. The bacteria immobilized webs were then tested for five times of reuse at an initial dye concentration of 100 mg/L, and found as potentially reusable with higher bacterial immobilization and faster dye removal capacities at the end of the test. Overall, these findings suggest that electrospun nanofibrous webs are available platforms for bacterial integration and the bacteria immobilized webs can be used as starting inocula for use in remediation of textile dyes in wastewater systems.
本研究介绍了通过将特定细菌固定在电纺纳米纤维网上制备用于去除纺织染料的新型生物复合材料及其应用。选择一种用于修复纺织染料的细菌分离株——密执安棒杆菌进行细菌固定，选择一种商业活性纺织染料——Setazol Blue BRF-X作为目标污染物，并选择电纺聚己内酯（PCL）和聚乳酸（PLA）纳米纤维聚合物网用于细菌整合。通过扫描电子显微镜（SEM）成像监测细菌在纳米纤维网上的粘附情况，并对分离出的细菌进行光密度（OD）测量。在电纺纳米纤维网上获得足够数量的固定化细菌后，使用等量的网样来测试染料去除能力。细菌/PCL网和细菌/PLA网在每个测试浓度（50、100和200mg/L）下均在48小时内显示出对Setazol Blue BRF-X染料的高效修复，其去除性能与游离细菌细胞非常相似。然后在初始染料浓度为100mg/L的条件下对固定有细菌的网进行五次重复使用测试，发现在测试结束时它们具有潜在的可重复使用性，且细菌固定量更高，染料去除速度更快。总体而言，这些发现表明电纺纳米纤维网是用于细菌整合的可用平台，固定有细菌的网可作为起始接种物用于废水系统中纺织染料的修复。
