Palermo M S, Giordano M, Olabuenaga S, Isturiz M A
Cell Immunol. 1985 Jul;93(2):438-46. doi: 10.1016/0008-8749(85)90148-0.
The effect of cyclophosphamide (Cy) on suppression of antibody-dependent cellular cytotoxicity (ADCC) by lymph node cells (LNC) was evaluated. The results show that the suppression of ADCC exerted by LNC was abrogated when mice had been treated with Cy. Moreover, it was shown that ADCC inhibition induced by LNC was mediated by soluble factor(s) and that treatment with a single dose of 200 mg/kg ip of Cy, significantly decreased its release. In addition, suppressor activity of normal LNC was enriched by depletion of adherent cells and was not affected by treatment with monoclonal anti-Thy 1.2 plus complement. These observations indicate that modulatory cells are nonadherent and lack characteristic T-cell markers. Thus, we conclude that this suppressor system, which normally controls ADCC activity, can be inhibited by treatment of mice with Cy and that this effect may explain the enhancement of ADCC observed in splenocytes of Cy-treated animals.
评估了环磷酰胺（Cy）对淋巴结细胞（LNC）抑制抗体依赖性细胞毒性（ADCC）的作用。结果显示，当用Cy处理小鼠时，LNC对ADCC的抑制作用被消除。此外，表明LNC诱导的ADCC抑制是由可溶性因子介导的，并且单次腹腔注射200mg/kg的Cy处理显著降低了其释放。另外，通过去除贴壁细胞富集了正常LNC的抑制活性，并且不受单克隆抗Thy 1.2加补体处理的影响。这些观察结果表明调节细胞是非贴壁的且缺乏特征性T细胞标志物。因此，我们得出结论，这种通常控制ADCC活性的抑制系统可通过用Cy处理小鼠而被抑制，并且这种作用可能解释了在Cy处理动物的脾细胞中观察到的ADCC增强现象。
