Wong T M, Greenberg M J, Tse S Y
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;81(1):175-9. doi: 10.1016/0742-8413(85)90111-2.
The molluscan neuropeptide, Phe-Met-Arg-Phe-NH2 (FMRFamide), the mammalian opioid peptide met-enkephalin, and their common analogues, met-enkephalin-Arg6-Phe7 (YGGFMRF) and Tyr-Gly-Gly-Phe-Met-Arg-Phe-amide (YGGFMRFamide), were injected into the lateral ventricle of the rat; the cardiovascular effects were studied. FMRFamide caused a rapid, transient elevation in blood pressure accompanied by a great increase in pulse pressure. These effects were followed by secondary increases in blood and pulse pressures. Met-enkephalin produced an initial reduction in blood pressure which was followed by a gradual increase at the higher of two test doses (300 nmole). Injection of YGGFMRF resulted in a gradual increase in blood pressure. This response resembled that to met-enkephalin. The initial response to YGGFMRFamide was similar to that to FMRFamide: increases in both blood and pulse pressures after injection. However, the secondary effect of YGGFMRFamide, a prolonged reduction in blood pressure, was not produced by FMRFamide. These results suggest that the initial excitatory cardiovascular responses may be due to the presence of the C-terminal amide. All of the cardiovascular effects of injecting these peptides into the lateral ventricle were abolished by pre-treatment with naloxone in a dose that, itself, produced no cardiovascular changes. In conclusion, these peptides seem to act via the naloxone sensitive opiate receptors in the rat brain.
将软体动物神经肽苯丙氨酸-蛋氨酸-精氨酸-苯丙氨酸-氨基（FMRF酰胺）、哺乳动物阿片肽甲硫氨酸脑啡肽及其共同类似物甲硫氨酸脑啡肽-精氨酸6-苯丙氨酸7（YGGFMRF）和酪氨酸-甘氨酸-甘氨酸-苯丙氨酸-甲硫氨酸-精氨酸-苯丙氨酸酰胺（YGGFMRF酰胺）注入大鼠侧脑室，研究其心血管效应。FMRF酰胺导致血压迅速短暂升高，同时脉压大幅增加。这些效应之后是血压和脉压的二次升高。甲硫氨酸脑啡肽最初使血压降低，在两个测试剂量中较高剂量（300纳摩尔）时随后逐渐升高。注射YGGFMRF导致血压逐渐升高。这种反应类似于对甲硫氨酸脑啡肽的反应。对YGGFMRF酰胺的初始反应与对FMRF酰胺的反应相似：注射后血压和脉压均升高。然而，YGGFMRF酰胺的二次效应，即血压的长期降低，FMRF酰胺并未产生。这些结果表明，最初的兴奋性心血管反应可能归因于C末端酰胺的存在。预先用纳洛酮处理，其剂量本身不会引起心血管变化，可消除将这些肽注入侧脑室所产生的所有心血管效应。总之，这些肽似乎通过大鼠脑中对纳洛酮敏感的阿片受体起作用。
