Murakami Tomohiko, Otsuki Shuhei, Nakagawa Kosuke, Okamoto Yoshinori, Inoue Tae, Sakamoto Yuki, Sato Hideki, Neo Masashi
1 Osaka Medical College, Osaka, Japan.
2 QOL Research Center Laboratory, Gunze Limited, Kyoto, Japan.
J Biomater Appl. 2017 Aug;32(2):150-161. doi: 10.1177/0885328217713631. Epub 2017 Jun 13.
The purpose of this study was to evaluate various types of meniscus scaffolds that mimic the meniscus structure, and to establish a novel cell-free meniscus scaffold with polyglycolic acid or poly-l-lactic acid. Four types of scaffolds were implanted into Japanese white rabbits: poly-l-lactic acid sponge poly-l-lactic acid, PGA-coated PLLA sponge, PGA lamination, and film-coated PGA lamination. Samples were harvested at 8 and 12 weeks after implantation, and a compression stress test was performed. The meniscus size and Ishida scores were evaluated for regenerated tissue. Immunohistochemistry was analyzed by anti-type I, II and X collagen antibodies to investigate the structure of the regenerated tissue, and by anti-iNOS antibody to investigate the inflammatory tissue of the meniscus. The cell nuclei of lymphocytes and foreign body multinucleated giant cells were counted in hematoxylin and eosin staining. Modified Mankin scores for cartilage degeneration were used for assessment after Safranin-O/Fast Green staining. The biomechanical test showed that l- and film-coated PGA lamination exhibited greater strength than s- and PGA-coated PLLA sponge. At 12 weeks, the size of meniscus and the Ishida score in implanted film-coated PGA lamination were improved significantly compared with the defect groups. The type II collagen staining intensity in the PGA lamination lamination is significantly higher than the defect at eight weeks. The staining intensity of iNOS and number of lymphocytes significantly increased in sponge poly-l-lactic acid at eight weeks, and increased in p-PLLA at 12 weeks. Foreign body multinucleated giant cells in implantation groups appeared, especially at eight weeks. The Mankin score for film-coated PGA lamination was significantly lower than for the defect at 12 weeks. Novel meniscal scaffolds especially PGA should possess not only biological but also biomechanical functions. In conclusions, film-coated PGA lamination was the beneficial property for meniscus scaffold from the points of better biomechanical function, good regeneration, and less inflammation with chondroprotective effects.
本研究的目的是评估模拟半月板结构的各种类型的半月板支架，并建立一种新型的聚乙醇酸或聚左旋乳酸无细胞半月板支架。将四种类型的支架植入日本白兔体内：聚左旋乳酸海绵聚左旋乳酸、聚乙醇酸涂层的聚左旋乳酸海绵、聚乙醇酸层叠材料和薄膜涂层的聚乙醇酸层叠材料。在植入后8周和12周采集样本，并进行压缩应力测试。对再生组织评估半月板大小和石田评分。通过抗I型、II型和X型胶原抗体进行免疫组织化学分析以研究再生组织的结构，通过抗诱导型一氧化氮合酶（iNOS）抗体研究半月板的炎症组织。在苏木精和伊红染色中对淋巴细胞和异物多核巨细胞的细胞核进行计数。在番红O/固绿染色后，使用改良的曼金软骨退变评分进行评估。生物力学测试表明，层叠和薄膜涂层的聚乙醇酸层叠材料比海绵和聚乙醇酸涂层的聚左旋乳酸海绵表现出更大的强度。在12周时，与缺损组相比，植入薄膜涂层聚乙醇酸层叠材料的半月板大小和石田评分显著改善。聚乙醇酸层叠材料在8周时II型胶原染色强度明显高于缺损处。iNOS的染色强度和淋巴细胞数量在8周时聚左旋乳酸海绵中显著增加，在12周时聚左旋乳酸海绵中增加。植入组出现异物多核巨细胞，尤其是在8周时。薄膜涂层聚乙醇酸层叠材料在12周时的曼金评分显著低于缺损处。新型半月板支架尤其是聚乙醇酸不仅应具有生物学功能，还应具有生物力学功能。总之，从更好的生物力学功能、良好的再生以及较少的炎症和软骨保护作用来看，薄膜涂层聚乙醇酸层叠材料对半月板支架具有有益特性。
