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植物激素串扰调控拟南芥根生长过程中 miR166/165s 的表达，靶向 Class III HD-ZIPs 和 KANADI 基因。

Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana.

机构信息

National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India.

出版信息

Sci Rep. 2017 Jun 13;7(1):3408. doi: 10.1038/s41598-017-03632-w.

DOI:10.1038/s41598-017-03632-w
PMID:28611467
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5469759/
Abstract

Both phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) transcription factors (TFs). However, their regulation through hormones remained poorly understood. Here, we show that several phytohormones dynamically regulate the spatio-temporal expression pattern of miR166/165 and target HD-ZIP IIIs in developing roots. Hormone signaling pathway mutants show differential expression pattern of miR166/165, providing further genetic evidence for multilayered regulation of these genes through phytohormones. We further show that a crosstalk of at least six different phytohormones regulate the miR166/165, their target HD-ZIP IIIs, and KANADI (KANs). Our results suggest that HD-ZIP IIIs mediated root development is modulated both transcriptionally through phytohormones and KANs, and post-transcriptionally by miR166/165 that in turn are also regulated by the phytohormonal crosstalk.

摘要

植物激素和非编码 microRNAs（miRNAs）在拟南芥根发育中都发挥着重要作用。成熟的 miR166/165s 来源于相关基因的前体转录本，通过靶向 III 类同源异型域亮氨酸拉链（HD-ZIP III）转录因子（TFs）来调节发育过程，包括叶片和根的模式形成。然而，它们通过激素的调节仍然知之甚少。在这里，我们表明，几种植物激素动态调节 miR166/165 的时空表达模式，并在发育中的根中靶向 HD-ZIP IIIs。激素信号通路突变体显示出 miR166/165 的差异表达模式，为这些基因通过植物激素进行多层次调节提供了进一步的遗传证据。我们进一步表明，至少六种不同的植物激素的相互作用调节 miR166/165、其靶标 HD-ZIP IIIs 和 KANADI（KANs）。我们的研究结果表明，HD-ZIP III 介导的根发育受到植物激素和 KANs 的转录调控，以及 miR166/165 的转录后调控，而 miR166/165 反过来也受到植物激素相互作用的调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/80f3546e6363/41598_2017_3632_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/13f4f2ca3b4b/41598_2017_3632_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/fde9f6b49a05/41598_2017_3632_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/2db331dbc494/41598_2017_3632_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/36065a8bf197/41598_2017_3632_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/0a6956d46591/41598_2017_3632_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/1247f4a01852/41598_2017_3632_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/80f3546e6363/41598_2017_3632_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/13f4f2ca3b4b/41598_2017_3632_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/fde9f6b49a05/41598_2017_3632_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/2db331dbc494/41598_2017_3632_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/36065a8bf197/41598_2017_3632_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/0a6956d46591/41598_2017_3632_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/1247f4a01852/41598_2017_3632_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cd4/5469759/80f3546e6363/41598_2017_3632_Fig7_HTML.jpg

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