Soma Sekhar M, Srinivasa Rao T, Chinnam B K, Subramanyam K V, Metta M, Mohammad Sharif N
Department of Veterinary Public Health and Epidemiology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Krishna (District), Andhra Pradesh 521102 India.
Department of Veterinary Microbiology, NTR College of Veterinary Science, Gannavaram, 521102 India.
Indian J Microbiol. 2017 Jun;57(2):250-252. doi: 10.1007/s12088-017-0649-1. Epub 2017 Apr 13.
is an emerging foodborne pathogen having zoonotic significance. Enterobacterial repetitive intergenic consensus (ERIC) PCR and repetitive sequence-based PCR (rep-PCR) analysis of a total of 41 isolates revealed a greater degree of genetic diversity. ERIC-PCR genotyping distinguished 14, 13 and 12 genotypes among 16, 13 and 12 isolates of , and , respectively. Rep-PCR genotyping distinguished 15, 12 and 11 genotypes among 16, 13 and 12 isolates of , and , respectively. The discriminatory power for ERIC and rep-PCR was found to be 0.997 and 0.996, respectively. Close clustering between isolates of animal and human origin are indicative of probable zoonotic significance.
是一种具有人畜共患病意义的新兴食源性病原体。对总共41株分离株进行肠杆菌重复基因间共识(ERIC)PCR和基于重复序列的PCR(rep-PCR)分析,结果显示出更大程度的遗传多样性。ERIC-PCR基因分型在、和的16株、13株和12株分离株中分别区分出14种、13种和12种基因型。Rep-PCR基因分型在、和的16株、13株和12株分离株中分别区分出15种、12种和11种基因型。发现ERIC和rep-PCR的鉴别力分别为0.997和0.996。动物源和人源分离株之间的紧密聚类表明可能具有人畜共患病意义。