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分离株的抗菌药敏性及基因组结构

Antimicrobial Susceptibility and Genomic Structure of Isolates.

作者信息

Hänel Ingrid, Hotzel Helmut, Tomaso Herbert, Busch Anne

机构信息

Institute of Bacterial Infections and Zoonoses (IBIZ), Friedrich Loeffler Institute, Jena, Germany.

出版信息

Front Microbiol. 2018 Dec 14;9:3067. doi: 10.3389/fmicb.2018.03067. eCollection 2018.

Abstract

spp. are considered the most common bacterial cause of foodborne gastroenteritis in the world. The family includes the genus with the three species , , and as emergent enteropathogens and potential zoonotic agents. Here, we characterized genome sequences of that were isolated from water poultry on farms in Germany. Isolates were cultured, identified by MALDI-TOF MS and identification was verified with PCR assays. Antibiotic susceptibility testing of isolates was carried out with erythromycin, ciprofloxacin, doxycycline, tetracycline, gentamicin, and streptomycin using the gradient strip method (-test). We also sequenced whole genomes and predicted antibiotic resistance determinants, virulence factors, performed a phylogenetic analysis to determine the genetic relatedness of these isolates and searched for plasmids.

摘要

某属细菌被认为是全球食源性肠胃炎最常见的细菌病因。该菌科包括某属,其中三种菌种,即[具体菌种1]、[具体菌种2]和[具体菌种3]作为新出现的肠道病原体和潜在的人畜共患病原体。在此,我们对从德国农场水禽中分离出的该属细菌的基因组序列进行了特征分析。分离菌株经培养后,通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)进行鉴定,并用聚合酶链反应(PCR)检测进行验证。使用梯度纸条法(E-test)对分离菌株进行了红霉素、环丙沙星、强力霉素、四环素、庆大霉素和链霉素的药敏试验。我们还对全基因组进行了测序,预测了抗生素耐药决定因素、毒力因子,进行了系统发育分析以确定这些分离菌株的遗传相关性,并搜索了质粒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e3/6302008/3e46f185b122/fmicb-09-03067-g001.jpg

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