Birckbichler P J, Upchurch H F, Patterson M K, Conway E
Hybridoma. 1985 Summer;4(2):179-86. doi: 10.1089/hyb.1985.4.179.
A cellular enzyme-linked immunosorbent assay was developed for estimating cellular transglutaminase in situ using a monoclonal antibody produced to tissue transglutaminase. The minimum level of detection of TGase was 2-5 ng. The enzyme was present in greater amounts in WI-38 and IMR90 cells than in their simian virus-transformed counterparts. The levels of TGase in the virus-transformed cells increased significantly when the cells were grown in the presence of sodium butyrate to induce enzyme activity. Staining of confluent WI-38 cells by indirect immunofluorescence using the monoclonal antibody showed microscopic fibers suggesting that the enzyme may be associated with detergent-insoluble components.
开发了一种细胞酶联免疫吸附测定法，用于使用针对组织转谷氨酰胺酶产生的单克隆抗体原位估计细胞转谷氨酰胺酶。转谷氨酰胺酶的最低检测水平为2 - 5 ng。WI - 38和IMR90细胞中该酶的含量比它们的猿猴病毒转化对应物中的含量更高。当细胞在丁酸钠存在下生长以诱导酶活性时，病毒转化细胞中转谷氨酰胺酶的水平显著增加。使用单克隆抗体通过间接免疫荧光对汇合的WI - 38细胞进行染色，显示出微观纤维，表明该酶可能与去污剂不溶性成分相关。
