采用夹心酶联免疫吸附测定系统对组织转谷氨酰胺酶进行定量分析。

Quantitation of tissue transglutaminase by a sandwich ELISA system.

作者信息

Fesus L, Arato G

出版信息

J Immunol Methods. 1986 Nov 20;94(1-2):131-6. doi: 10.1016/0022-1759(86)90225-5.

Abstract

A sandwich ELISA system has been developed to quantitate transglutaminase in human cell extracts. It utilizes affinity-purified rabbit anti-human transglutaminase as the capture antibody and a mouse monoclonal anti-transglutaminase (Birckbichler et al., 1985) as the indicator antibody (together with peroxidase-labeled anti-mouse immunoglobulin). The sensitivity of the assay was less than 1.0 ng/mg cellular protein. Significantly higher concentrations of the enzyme were found in resting versus proliferating or transformed fibroblasts in good agreement with previous activity measurements. The levels of transglutaminase in normal T and B lymphocytes, malignant lymphoid cells and monocytes were also determined.

摘要

已开发出一种夹心酶联免疫吸附测定（ELISA）系统，用于定量人细胞提取物中的转谷氨酰胺酶。它利用亲和纯化的兔抗人转谷氨酰胺酶作为捕获抗体，以及小鼠抗转谷氨酰胺酶单克隆抗体（Birckbichler等人，1985年）作为指示抗体（与过氧化物酶标记的抗小鼠免疫球蛋白一起使用）。该测定的灵敏度低于1.0纳克/毫克细胞蛋白。与先前的活性测量结果高度一致，在静息成纤维细胞中发现的该酶浓度明显高于增殖或转化的成纤维细胞。还测定了正常T和B淋巴细胞、恶性淋巴样细胞和单核细胞中转谷氨酰胺酶的水平。