Urbaniak Monika, Waśkiewicz Agnieszka, Koczyk Grzegorz, Błaszczyk Lidia, Stępień Łukasz
Plant-Pathogen Interaction Team, Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Department of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland.
J Fungi (Basel). 2020 Nov 15;6(4):288. doi: 10.3390/jof6040288.
Beauvericin (BEA) is a cyclodepsipeptide mycotoxin, showing insecticidal, antibiotic and antimicrobial activities, as well as inducing apoptosis of cancer cell lines. BEA can be produced by multiple fungal species, including saprotrophs, plant, insect and human pathogens, particularly belonging to , and genera. The ability of species to produce BEA was until now uncertain. Biosynthesis of BEA is governed by a non-ribosomal peptide synthase (NRPS), known as beauvericin synthase (BEAS), which appears to present considerable divergence among different fungal species. In the present study we compared the production of beauvericin among and strains using UPLC methods. fragments were sequenced and analyzed to examine the level of the gene's divergence between these two genera and confirm the presence of active copy in . Seventeen strains of twelve species were studied and phylogenetic analysis showed distinctive grouping of and strains. The highest producers of beauvericin were and . strains of three species (, , ) were minor BEA producers. The study showed beauvericin production by and species and high variance of the non-ribosomal peptide synthase gene among fungal species from the order.
白僵菌素（BEA）是一种环缩肽霉菌毒素，具有杀虫、抗菌和抗微生物活性，还能诱导癌细胞系凋亡。BEA可由多种真菌产生，包括腐生菌、植物、昆虫和人类病原体，特别是属于 、 和 属的真菌。 属物种产生BEA的能力至今尚不确定。BEA的生物合成由一种非核糖体肽合成酶（NRPS）控制，即白僵菌素合成酶（BEAS），不同真菌物种中的BEAS似乎存在很大差异。在本研究中，我们使用超高效液相色谱法比较了 和 菌株中白僵菌素的产量。对 片段进行测序和分析，以检测这两个属之间该基因的差异水平，并确认 在 中存在活性拷贝。研究了12个物种的17个菌株，系统发育分析显示 和 菌株有明显的分组。白僵菌素产量最高的是 和 。三个物种（ 、 、 ）的 菌株是少量白僵菌素生产者。该研究表明 和 物种可产生白僵菌素，且 目真菌物种中非核糖体肽合成酶基因存在高度变异性。
